Rabbit creatine kinase MB isoenzyme (CK-MB) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔肌酸激酶同工酶MB(CK-MB)酶聯免疫試劑盒（CSB-EQ027653RB）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CK-MB含量。CK-MB即肌酸激酶同工酶，主要存在于心肌中。當心肌損傷時，CK - MB會釋放入血，其水平變化可反映心肌損傷程度。研究多圍繞其作為心肌損傷早期敏感標志物展開，監測它對急性心肌梗死等疾病的診斷、治療及預后評估有重要意義。試劑盒檢測范圍為1.56 mU/mL-100 mU/mL，適用于心血管疾病機制研究、藥物心臟毒性評估及動物模型中心肌損傷的動態監測；適用于心肌缺血再灌注損傷、藥物干預效果評價、病理模型建立等科研場景，助力心血管相關疾病的基礎研究與轉化探索本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

CK-MB
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 mU/mL-100 mU/mL
靈敏度：

0.39 mU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-93
1:2	Average %	95
	Range %	90-99
1:4	Average %	94
	Range %	89-100
1:8	Average %	102
	Range %	96-107

回收率：

The recovery of rabbit CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	83-96
EDTA plasma (n=4)	92	88-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.