Mouse Collagen Type Ⅲ,Col Ⅲ ELISA KIT

產(chǎn)品詳情

文獻(xiàn)引用 (8) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠Ⅲ型膠原(Col Ⅲ)酶聯(lián)免疫試劑盒（CSB-E07925m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的Col Ⅲ含量。Col Ⅲ即Ⅲ型膠原蛋白，它是細(xì)胞外基質(zhì)重要成分，在維持組織器官結(jié)構(gòu)和功能上起關(guān)鍵作用。研究發(fā)現(xiàn)其異常表達(dá)與多種疾病相關(guān)，如纖維化疾病等。機(jī)制上，可能通過影響細(xì)胞黏附、遷移等行為，參與疾病發(fā)生發(fā)展過程。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，能夠滿足纖維化疾病機(jī)制研究、藥物干預(yù)效果評估等科研需求。可廣泛應(yīng)用于心血管疾病模型、器官纖維化模型等研究中 Col Ⅲ 動態(tài)變化的監(jiān)測，為探索纖維化相關(guān)分子機(jī)制提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
縮寫：

Col Ⅲ
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	89
	Range %	82-96
1:40	Average %	98
	Range %	94-102
1:80	Average %	97
	Range %	90-104
1:160	Average %	86
	Range %	82-90

回收率：

The recovery of mouse Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-99
EDTA plasma (n=4)	100	96-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.