Pig Collagen Type Ⅲ,Col Ⅲ ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬Ⅲ型膠原(Col Ⅲ)酶聯免疫試劑盒（CSB-E06776p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Col Ⅲ含量。Col Ⅲ（膠原蛋白Ⅲ）是細胞外基質的主要成分之一，參與維持肺動脈管壁的彈性和完整性。在肺動脈高壓（PAH）等疾病中，TGF-β1等因子促進PASMCs外膠原沉積，導致肺血管重構。低濃度紫杉醇（PTX）可通過抑制TGF-β1/Smad3信號通路，降低COL Ⅲ表達，從而逆轉纖維化進程。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，適用于探究豬模型在纖維化機制、心血管疾病或皮膚再生等領域的科研需求，為組織工程、病理模型構建及分子機制研究提供可靠數據支持，是基礎研究與轉化醫學中膠原代謝相關課題的理想工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
縮寫：

Col Ⅲ
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

1.56 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	91-103
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	93
	Range %	89-95

回收率：

The recovery of pig Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	97	92-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.