Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人Ⅲ型膠原(Col Ⅲ)酶聯免疫試劑盒（CSB-E04799h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Col Ⅲ含量。人Ⅲ型膠原（Col Ⅲ）是人體皮膚中重要的膠原蛋白類型，主要存在于嬰兒皮膚和血管內膜。其研究背景涉及膠原蛋白的生物學功能，如支撐皮膚結構、促進細胞遷移和修復。研究機制則聚焦于其三螺旋結構、細胞粘附性及其在皮膚修復和抗衰老中的作用。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL，適用于體外研究Col Ⅲ在皮膚老化、肝纖維化、心血管疾病或組織工程修復等領域的表達調控，可為探索纖維化疾病分子機制或評估抗纖維化藥物效果提供數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
縮寫：

Col Ⅲ
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 ng/mL-800 ng/mL
靈敏度：

3.12 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-96
1:2	Average %	102
	Range %	97-105
1:4	Average %	92
	Range %	85-98
1:8	Average %	97
	Range %	91-102

回收率：

The recovery of human Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	80-94
EDTA plasma (n=4)	97	92-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.