Mouse 4-Hydroxynonenal (HNE)ELISA Kit

Mouse 4-Hydroxynonenal (HNE)ELISA Kit

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文獻引用 (13) 說明書下載

中文名稱：

小鼠4-羥基壬烯酸(HNE)酶聯免疫試劑盒
貨號：

CSB-E13412m
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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引用文獻
相關產品

產品描述：

小鼠4-羥基壬烯酸(HNE)酶聯免疫試劑盒（CSB-E13412m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的HNE含量。HNE即人中性粒細胞彈性蛋白酶，在炎癥反應等生理病理過程中扮演重要角色。其過度表達與肺氣腫、急性呼吸窘迫綜合征等疾病密切相關。研究主要圍繞抑制其活性展開，期望開發出能治療相關疾病的藥物，為臨床治療提供新方向。試劑盒檢測范圍為15.6 pmol/mL-1000 pmol/mL，本試劑盒可應用于氧化損傷相關研究領域，如代謝性疾病模型、心血管功能異常或神經退行性病變等科研場景中，通過檢測血清或血漿樣本的HNE水平，評估氧化應激程度及其在疾病進展中的作用機制，為實驗動物模型的病理機制探索提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

HNE
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

15.6 pmol/mL-1000 pmol/mL
靈敏度：

3.9 pmol/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	96
	Range %	91-103
1:4	Average %	94
	Range %	90-99
1:8	Average %	94
	Range %	88-98

回收率：

The recovery of mouse HNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-97
EDTA plasma (n=4)	94	90-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pmol/ml	OD1	OD2	Average	Corrected
1000	2.608	2.555	2.582	2.392
500	1.939	1.897	1.918	1.728
250	1.227	1.212	1.220	1.030
125	0.821	0.759	0.790	0.600
62.5	0.468	0.450	0.459	0.269
31.2	0.331	0.328	0.330	0.140
15.6	0.278	0.271	0.275	0.085
0	0.192	0.188	0.190

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.