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中文名稱：人p物質(SP)酶聯免疫試劑盒
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貨號：CSB-E08357h
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規格：96T/48T
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價格：￥3200/￥2500
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促銷：
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其他：
產品詳情
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產品描述：人p物質(SP)酶聯免疫試劑盒（CSB-E08357h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SP含量。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于神經生物學、免疫調節機制等基礎研究，特別在疼痛模型評估、神經炎癥研究或胃腸道功能實驗中，可為探索SP在病理生理過程中的動態變化提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：N/A
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縮寫：SP
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：6.25 pg/mL-400 pg/mL
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靈敏度：1.56 pg/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Neuroscience
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 92-100 1:2 Average % 102 Range % 98-105 1:4 Average % 89 Range % 85-96 1:8 Average % 104 Range % 100-108 -
回收率：
The recovery of human SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 86 82-92 EDTA plasma (n=4) 85 81-90 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 400 2.439 2.489 2.464 2.290 200 1.933 1.978 1.956 1.782 100 1.285 1.298 1.292 1.118 50 0.799 0.782 0.791 0.617 25 0.475 0.468 0.472 0.298 12.5 0.376 0.394 0.385 0.211 6.25 0.316 0.308 0.312 0.138 0 0.175 0.173 0.174 -
數據處理：
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貨期：3-5 working days
引用文獻
- Recurrent infections drive persistent bladder dysfunction and pain via sensory nerve sprouting and mast cell activity BW Hayes,Science immunology,2024
- Influence of video display terminal use and meibomian gland dysfunction on the ocular surface and tear neuromediators AA Bilgic,International Ophthalmology,2022
- Network Pharmacology Analysis of Hewei Jiangni Granule for Gastroesophageal Reflux Disease and Experimental Verification of Its Anti-Neurogenic Inflammation Mechanism Y Cheng,Drug design, development and therapy,2022
- Testing feasibility of traditional Malay massage compared to relaxation for patients with non-specific low back pain: A pilot randomized-controlled trial N Sejari,European Journal of Integrative Medicine,2020
- MMP1/PAR1/SP/NK1R paracrine loop modulates early perineural invasion of pancreatic cancer cells Chumei Huang.et al,Theranostics,2018
- The immediate effect of traditional Malay massage on substance P, inflammatory mediators, pain scale and functional outcome among patients with low back pain: study protocol of a randomised controlled trial. Nurhanisah Sejari.et al,BMC Complementary and Alternative Medicine,2016
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