Mouse Substance P,SP ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 評(píng)價(jià) (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠P物質(zhì)(SP)酶聯(lián)免疫試劑盒（CSB-E08359m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的SP含量。試劑盒檢測(cè)范圍為3.12 pg/mL-200 pg/mL，廣泛用于基礎(chǔ)科研領(lǐng)域，如探究神經(jīng)系統(tǒng)疾病模型中SP的分泌動(dòng)態(tài)、評(píng)估藥物對(duì)神經(jīng)肽表達(dá)的調(diào)控作用，或解析炎癥反應(yīng)與神經(jīng)信號(hào)通路的關(guān)聯(lián)機(jī)制，為神經(jīng)生物學(xué)、免疫學(xué)及藥理學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

SP
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Neuroscience
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	92-104
1:200	Average %	94
	Range %	89-98
1:400	Average %	89
	Range %	86-96
1:800	Average %	94
	Range %	89-97

回收率：

The recovery of mouse SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	86	82-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：血清

樣品信息：小鼠

稀釋比：其他 1:200

產(chǎn)品評(píng)價(jià)：我用CSB-E08359m檢測(cè)正常血清，其od值為0.7/0.604/0.559/0.639/0.577/0.671

By 張老師