Human Angiotensin 1-7(Ang1-7)ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (8) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人血管緊張素1-7（Ang1-7）酶聯(lián)免疫試劑盒（CSB-E14242h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液樣本中的Ang1-7含量。Ang1-7是一種血管緊張素，具有血管舒張作用，影響心血管器官如心臟、血管和腎臟。研究顯示，Ang1-7通過降低ERK1/2MAPK活性，調(diào)節(jié)心血管功能，并在肺纖維化、肝癌轉(zhuǎn)移等疾病中發(fā)揮作用。試劑盒檢測(cè)范圍為7.8 pg/mL-500 pg/mL，適用于心血管疾病機(jī)制研究、藥物干預(yù)效果評(píng)估、細(xì)胞模型中Ang1-7分泌水平檢測(cè)等科研領(lǐng)域，尤其適合低豐度樣本的定量分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Ang1-7
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates
檢測(cè)范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	80-105
1:2	Average %	96
	Range %	85-109
1:4	Average %	105
	Range %	92-114
1:8	Average %	95
	Range %	82-110

回收率：

The recovery of human Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-110
EDTA plasma (n=4)	99	90-108

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.