Dog angiotensin 1-7 (Ang1-7) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

犬血管緊張素1-7(Ang1-7)酶聯(lián)免疫試劑盒（CSB-EQ027505DO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、組織培養(yǎng)上清液樣本中的Ang1-7含量。Ang1-7是重要靶點(diǎn)。它屬于腎素-血管緊張素系統(tǒng)成員。其研究機(jī)制主要體現(xiàn)在可通過(guò)結(jié)合Mas受體，發(fā)揮舒張血管、降低血壓、抑制炎癥和纖維化等作用，在心血管疾病、腎臟疾病等研究領(lǐng)域有潛在治療價(jià)值。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，研究者可將其應(yīng)用于犬類心血管疾病模型、腎臟功能研究、代謝綜合征機(jī)制探索，以及藥物干預(yù)或基因調(diào)控對(duì)Ang1 - 7通路影響的體外實(shí)驗(yàn)分析，為深入解析RAS系統(tǒng)在病理生理過(guò)程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
縮寫：

Ang1-7
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates, cell culture supernates
檢測(cè)范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-92
1:2	Average %	100
	Range %	97-103
1:4	Average %	86
	Range %	83-89
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of dog Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	91	85-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.