Horse Beta-Endorphin,β-EP ELISA Kit

說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

馬β內(nèi)啡肽(β-EP)酶聯(lián)免疫試劑盒（CSB-E06831Eq）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的β-EP含量。β-EP是重要靶點。它屬內(nèi)源性阿片肽，廣泛分布于中樞和外周。其參與痛覺調(diào)節(jié)、情緒調(diào)控等生理過程。研究機(jī)制上，主要通過與阿片受體結(jié)合發(fā)揮作用，深入探索其機(jī)制有助于開發(fā)治療疼痛、精神疾病等的藥物。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，適用于科研領(lǐng)域探究馬科動物在應(yīng)激狀態(tài)、慢性疼痛模型、神經(jīng)系統(tǒng)疾病或免疫調(diào)節(jié)研究中的β-EP動態(tài)變化，為揭示其生理功能及分子機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

beta-Endorphin, beta-EP
縮寫：

β-EP
種屬：

Equus caballus (Horse)
樣本類型：

serum, plasma
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	90-111
1:2	Average %	105
	Range %	102-109
1:4	Average %	93
	Range %	90-97
1:8	Average %	88
	Range %	85-93

回收率：

The recovery of horse β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-105
EDTA plasma (n=4)	91	90-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.