Bovine Beta-Endorphin,β-EP ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛β內啡肽(β-EP)酶聯免疫試劑盒（CSB-E13051B）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的β-EP含量。β - EP即β - 內啡肽，是人體自身產生的具有強大鎮痛作用的內源性活性物質。其研究機制主要圍繞它與體內阿片受體結合，調節痛覺傳導、情緒等生理過程，在鎮痛、精神疾病等領域研究較多，對理解人體生理和開發相關療法有重要意義。試劑盒檢測范圍為46.88 pg/mL-3000 pg/mL，可為神經生物學、疼痛機制研究或應激反應動物模型提供數據支持，例如探究藥物干預、環境刺激對β - EP表達水平的影響；適用于牛源樣本的體外定量分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

beta-Endorphin, beta-EP
縮寫：

β-EP
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

46.88 pg/mL-3000 pg/mL
靈敏度：

11.72 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-99
1:2	Average %	84
	Range %	80-88
1:4	Average %	92
	Range %	88-97
1:8	Average %	90
	Range %	85-94

回收率：

The recovery of bovine β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-104
EDTA plasma (n=4)	92	88-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.