Rat Beta-Endorphin,β-EP ELISA Kit

產(chǎn)品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠β內(nèi)啡肽(β-EP)酶聯(lián)免疫試劑盒（CSB-E08206r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的β-EP含量。β-內(nèi)啡肽（β-endorphin）是一種內(nèi)源性阿片肽，由垂體合成并儲存在丘腦、垂體等組織中。它通過作用于μ受體，調(diào)節(jié)生理活動，并參與成癮、疼痛和應(yīng)激反應(yīng)等生理過程。研究顯示，β-EP水平與多種疾病狀態(tài)相關(guān)，如成癮、疼痛和神經(jīng)源性炎癥等，其具體作用機制尚需進一步研究。試劑盒檢測范圍為10 pg/mL-2000 pg/mL，該產(chǎn)品可應(yīng)用于神經(jīng)生物學(xué)研究、疼痛機制探索、應(yīng)激反應(yīng)模型評估等科研領(lǐng)域，尤其適合分析不同實驗條件下β - EP在體液或組織中的動態(tài)變化，為動物模型中的神經(jīng)遞質(zhì)調(diào)控研究提供可靠檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

beta-Endorphin, beta-EP
縮寫：

β-EP
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

10 pg/mL-2000 pg/mL
靈敏度：

5 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-90
1:2	Average %	97
	Range %	91-105
1:4	Average %	94
	Range %	90-98
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of rat β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	90	84-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.