Dog Interleukin-8,IL-8 ELISA Kit

Dog Interleukin-8,IL-8 ELISA Kit

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中文名稱：

犬白介素8(IL-8/CXCL8)酶聯免疫試劑盒
貨號：

CSB-E08052c
規格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

犬白介素8(IL-8/CXCL8)酶聯免疫試劑盒（CSB-E08052c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的CXCL8含量。CXCL8作為一個靶點，它屬于趨化因子家族，與炎癥反應、細胞遷移等密切相關。研究發現，它可以通過與特定受體結合，激活下游信號通路，在腫瘤、自身免疫病等疾病發展進程中發揮作用，是當前藥物研發等領域的重要研究對象。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，靈敏度為0.146 ng/mL。本試劑盒通過高靈敏度抗體組合實現0.312 ng/mL至20 ng/mL的寬泛檢測范圍，可精準量化低豐度目標蛋白，滿足科研場景中細胞因子動態監測、炎癥模型評估或藥物干預效果分析等需求。其操作流程兼容常規實驗室設備，適用于獸醫基礎研究、實驗動物模型開發及生物制劑研發等領域，為探索犬類免疫系統功能及相關疾病機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
縮寫：

CXCL8
Uniprot No.：

P41324
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.146 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	99
	Range %	95-105
1:4	Average %	88
	Range %	85-93
1:8	Average %	90
	Range %	86-97

回收率：

The recovery of dog IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-94
EDTA plasma (n=4)	88	85-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	3.221	3.295	3.258	3.157
10	2.523	2.711	2.617	2.516
5	1.791	1.930	1.861	1.760
2.5	1.035	1.045	1.040	0.939
1.25	0.466	0.485	0.476	0.375
0.625	0.249	0.259	0.254	0.153
0.312	0.157	0.167	0.162	0.061
0	0.103	0.099	0.101

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Synergistic Action of IL-8 and Bone Marrow Concentrate on Cartilage Regeneration Through Upregulation of Chondrogenic Transcription Factors Dong Suk Yoon. et al,Tissue Engineering Part A.,2016

最新研究進展：

IL8，全稱為白細胞介素8，是一種由免疫細胞產生的炎癥介質。IL8通過結合CXCR1和CXCR2等受體調節免疫細胞的趨化和活化，參與多種炎癥反應的發生和發展。近年來的研究表明，IL8在腫瘤的發生和轉移中發揮重要作用。例如，IL8的高表達與乳腺癌、結直腸癌等多種腫瘤的不良預后相關。
功能：

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
基因功能參考文獻：
1. Analysis of ROC suggests the use of serum levels of MCP-1 and IL-7 as predictors of the occurrence of complications with an AUC of 0.906 and 0.896 respectively and linear combinations of MCP-1, KC-Like, IL-7 and GM-CSF with values up to AUC = 0.983 PMID: 29304171
2. These observations indicate that the individual activation of ERK2 and JNK1 pathways contributes to TNF-alpha-induced IL-8 expression in synovial fibroblasts. PMID: 28806729
3. data suggest that CCL2 acts as a PMNs chemotactic factor as well as CXCL8, and both CCL2 and CXCL8 facilitate the infiltration of PMNs into the joints of idiopathic polyarthritis PMID: 27863550
4. The study findings suggest that both CCL2 and CXCL8 are involved in the pathogenesis of canine idiopathic pulmonary fibrosis. PMID: 26231926
5. High CXCL8 blood concentrations might be related to the breed predisposition of the West Highland white terrier for canine idiopathic pulmonary fibrosis. PMID: 26267090
6. IL-8-positive macrophages were significantly increased in large polyps compared to controls PMID: 24148828
7. hemangiosarcoma derived IL-8 may play a role in tumor development by modulating the tumor microenvironment PMID: 24582862
8. Data indicate that the Leishmania braziliensis antigens plus saponin (LBSap) vaccine induced high levels of IL-12, IL-10, CCL4, CCL5 and CXCL8 expression within 48 hours. PMID: 23911411
9. serum and tumor levels of IL-8 and IL-10 in dogs bearing benign and malignant mammary tumors, including dogs with inflammatory mammary cancer, for a better understanding of this disease PMID: 23351639
10. Sixty-one percent (28/46) of the samples from canine stifle osteoarthritis had IL-8 mRNA expression in contrast to 4% (1/24) in the control stifle joints PMID: 16102844
11. Adipose tissue-derived mesenchymal stem cells expressed the mRNA of transforming growth factor beta (TGF-beta), IL-6, IL-8, CCL2, CCL5, VEGF,HGF, tissue inhibitor metalloproteinase-1/2, and cyclooxygenase-2 but not that of IL-10. PMID: 18717642
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亞細胞定位：

Secreted.
蛋白家族：

Intercrine alpha (chemokine CxC) family
數據庫鏈接：

KEGG: cfa:403850

STRING: 9615.ENSCAFP00000004516

UniGene: Cfa.3510

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.