Dog Complement 3(C3)ELISA Kit

Dog Complement 3(C3)ELISA Kit

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中文名稱：

犬補(bǔ)體蛋白3(C3)酶聯(lián)免疫試劑盒
貨號：

CSB-EL003888DO
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

犬補(bǔ)體蛋白3(C3)酶聯(lián)免疫試劑盒（CSB-EL003888DO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C3含量。C3是一種重要靶點(diǎn)。它在補(bǔ)體系統(tǒng)中扮演關(guān)鍵角色，參與免疫防御等生理過程。研究發(fā)現(xiàn)其異常激活與多種疾病相關(guān)，如自身免疫病、腎臟疾病等。科研人員探究其激活機(jī)制，期望通過干預(yù)C3來開發(fā)新的治療手段。試劑盒檢測范圍為0.78 μg/mL-50 μg/mL，本試劑盒適用于實(shí)驗(yàn)動(dòng)物模型研究、免疫病理機(jī)制探索及藥物干預(yù)效果評價(jià)等科研場景，例如通過檢測感染性疾病或免疫相關(guān)疾病模型中C3的濃度變化，揭示補(bǔ)體系統(tǒng)在犬類疾病發(fā)生發(fā)展中的作用，為獸醫(yī)學(xué)研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

AHUS5, ARMD9, ASP, CPAMD1, acylation-stimulating protein cleavage product|complement component C3
縮寫：

C3
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 μg/mL-50 μg/mL
靈敏度：

0.195 μg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	99
	Range %	95-102
1:200	Average %	95
	Range %	81-89
1:400	Average %	92
	Range %	88-96
1:800	Average %	101
	Range %	98-103

回收率：

The recovery of dog C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-97
EDTA plasma (n=4)	89	85-93

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
50	0.075	0.079	0.077
25	0.130	0.136	0.133
12.5	0.206	0.204	0.205
6.25	0.322	0.332	0.327
3.12	0.543	0.538	0.541
1.56	0.851	0.878	0.865
0.78	1.163	1.198	1.181
0	2.014	2.036	2.025

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Serum concentrations of complement C3 and C4 in dogs with idiopathic epilepsy J Zhou,Journal of veterinary internal medicine,2024

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.