Dog Complement 3(C3)ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

犬補體蛋白3(C3)酶聯免疫試劑盒（CSB-EL003888DO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C3含量。C3是一種重要靶點。它在補體系統中扮演關鍵角色，參與免疫防御等生理過程。研究發現其異常激活與多種疾病相關，如自身免疫病、腎臟疾病等。科研人員探究其激活機制，期望通過干預C3來開發新的治療手段。試劑盒檢測范圍為0.78 μg/mL-50 μg/mL，本試劑盒適用于實驗動物模型研究、免疫病理機制探索及藥物干預效果評價等科研場景，例如通過檢測感染性疾病或免疫相關疾病模型中C3的濃度變化，揭示補體系統在犬類疾病發生發展中的作用，為獸醫學研究提供可靠的數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

AHUS5, ARMD9, ASP, CPAMD1, acylation-stimulating protein cleavage product|complement component C3
縮寫：

C3
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 μg/mL-50 μg/mL
靈敏度：

0.195 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	99
	Range %	95-102
1:200	Average %	95
	Range %	81-89
1:400	Average %	92
	Range %	88-96
1:800	Average %	101
	Range %	98-103

回收率：

The recovery of dog C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-97
EDTA plasma (n=4)	89	85-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.