ROBO1

ROBO1，全稱為Roundabout homolog 1，在神經發育和癌癥中扮演著重要角色。它最初在果蠅中被發現，與Slit2配體共同作用，引導神經軸突生長方向，防止其跨過體軸中線形成紊亂。ROBO1蛋白也被稱為Dutt1，與多種癌癥的發生發展相關，如肝癌和結直腸癌。研究發現，ROBO1蛋白在肝癌細胞中常有過表達現象，且其表達水平與腫瘤的分期和級別無明顯關聯。此外，ROBO1蛋白在乳腺癌和腎細胞癌中也會被高度甲基化，提示其可能是一個抑癌基因。然而，在肝癌中，ROBO1蛋白卻呈現過表達狀態，這可能與腫瘤微環境中的信號通路變化有關。目前，針對ROBO1蛋白的藥物研發尚處于早期階段，但已有研究探索其作為潛在治療靶點的可能性。例如，有研究通過RNA干擾技術敲低ROBO1蛋白表達，發現可以顯著抑制肝癌細胞的遷移和侵襲能力，并降低其生長速度和轉移能力。這些發現提示ROBO1蛋白可能成為肝癌治療的新靶點，值得進一步研究。

熱銷產品

Recombinant Human Roundabout homolog 1 (ROBO1), partial (Active) (CSB-MP896760HU)

驗證數據

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA.Immobilized Human ROBO1 at 2 μg/ml can bind Anti-ROBO1 recombinant antibody (CSB-RA896760MA1HU).The EC₅₀ is 0.7363-0.9105 ng/mL.

The purity of ROBO1 was greater than 90% as determined by SEC-HPLC

ROBO1 Recombinant Monoclonal Antibody (CSB-RA896760MA1HU)

驗證數據

IHC image of CSB-RA896760MA1HU diluted at 1:30 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA896760MA1HU diluted at 1:30 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of A549 cell with CSB-RA896760MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescence staining of Hela cell with CSB-RA896760MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Overlay Peak curve showing Hela cells surface stained with CSB-RA896760MA1HU (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,025 events was performed.

ROBO1 Antibody (CSB-PA896760LA01HU)

驗證數據

Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: ROBO1 antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 181, 182, 177, 176, 170 kDa
Observed band size: 181 kDa

Immunofluorescent analysis of Hela cells using CSB-PA896760LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Human Roundabout homolog 1(ROBO1) ELISA kit (CSB-EL020054HU)

驗證數據

貨號：CSB-EL020054HU

規格：96T/48T

靈敏度：0.039 ng/mL

檢測范圍：0.156 ng/mL-10 ng/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ROBO1 Antibody

ROBO1 Protein

ROBO1 ELISA Kit

ROBO1 Antibodies

ROBO1 for Homo sapiens (Human)

產品貨號	產品名稱	種屬反應性	應用類型
CSB-PA020054GA01HU	ROBO1 Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA896760LA01HU	ROBO1 Antibody	Human	ELISA, WB, IF
CSB-PA896760LB01HU	ROBO1 Antibody, HRP conjugated	Human	ELISA
CSB-PA896760LC01HU	ROBO1 Antibody, FITC conjugated	Human
CSB-PA896760LD01HU	ROBO1 Antibody, Biotin conjugated	Human	ELISA
CSB-RA896760MA1HU	ROBO1 Recombinant Monoclonal Antibody	Human	ELISA, IHC, IF, FC

ROBO1 Proteins

ROBO1 Proteins for Homo sapiens (Human)

產品貨號	產品名稱	來源
CSB-YP896760HU CSB-EP896760HU CSB-BP896760HU CSB-EP896760HU-B	Recombinant Human Roundabout homolog 1 (ROBO1), partial	Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli
CSB-MP896760HU	Recombinant Human Roundabout homolog 1 (ROBO1), partial (Active)	Mammalian cell