Recombinant Human Roundabout homolog 1 (ROBO1), partial (Active)

^{In
Stock}

貨號：

CSB-MP896760HU
說明書：

MSDS Datasheet
規格：

￥828
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA.Immobilized Human ROBO1 at 2 μg/ml can bind Anti-ROBO1 recombinant antibody (CSB-RA896760MA1HU).The EC₅₀ is 0.7363-0.9105 ng/mL. Biological Activity Assay

The purity of ROBO1 was greater than 90% as determined by SEC-HPLC
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
內毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

Measured by its binding ability in a functional ELISA.Immobilized Human ROBO1 at 2 μg/mL can bind Anti-ROBO1 recombinant antibody (CSB-RA896760MA1HU).The EC50 is 0.7363-0.9105 ng/mL.
基因名：

ROBO1
Uniprot No.：

Q9Y6N7
別名：

Roundabout homolog 1; Deleted in U twenty twenty; H-Robo-1; ROBO1; DUTT1
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

Mammalian cell
分子量：

97.0 kDa
表達區域：

26-897aa
氨基酸序列

QLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQP
蛋白標簽：

C-terminal 10xHis-tagged
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development.
基因功能參考文獻：
1. Robo1 expression might counteract migration and also radiation-induced migration of glioblastoma cells, a process that might be connected to mesenchymal-epithelial transition. PMID: 29864155
2. the expression of hsa_circRNA0054633 has a protective effect against high glucoseinduced endothelial cell dysfunction by targeting ROBO1 and HO1. PMID: 29693114
3. Overexpression of miR-218 and inhibition of Robo1 reduced the number of the invased cells of HCC4006. PMID: 28738960
4. miR-218 inhibits human lung adenocarcinoma cell migration and invasion via the suppression of Ecop and Robo1 expression PMID: 28936884
5. we show that variants in ROBO1 are associated with CHD involving septal defects and tetralogy of Fallot. PMID: 28592524
6. SLIT2/ROBO1 signalling may regulate trophoblast differentiation and invasion causing restricting beta human chorionic gonadotrophin (beta-hCG) production, shallow trophoblast invasion and inhibiting placental angiogenesis in missed and threatened miscarriage during the first trimester. PMID: 28485101
7. The findings indicate that the migration of human neural progenitor cells from the fetal subventricular zone to the olfactory bulb is partially regulated by the Slit2-Robo1 axis. PMID: 28406573
8. These findings offer direct evidence supporting ROBO1-callosum association in humans and also provide valuable insight into the functions of ROBO1 and the gene-to-brain mechanisms that underlie human reading. PMID: 28240421
9. Differential expression levels and methylation status of ROBO1 in mantle cell lymphoma and chronic lymphocytic leukaemia. PMID: 28004534
10. The alteration of Slit2 and Robo1 expression in the retinas of diabetic rats and patients with proliferative diabetic retinopathy suggests a role for the Slit-Robo signal in the various stages diabetic retinopathy. PMID: 28973045
11. Results demonstrate that human ROBO1 may be involved in the regulation of the structure and connectivity of posterior part of corpus callosum. PMID: 27240594
12. ROBO1 gene mutation is responsible for the development of pituitary stalk interruption syndrome. PMID: 28402530
13. human placental multipotent mesenchymal stromal cell express Slit2 and both Robo1 and Robo4 are present in human umbilical vein endothelial cells. PMID: 26745454
14. Slit2-Robo1 signaling promoted the adhesion, invasion and migration of tongue carcinoma cells by upregulating the expression levels of MMP2 and MMP9 and, downregulating the expression of E-cadherin. PMID: 27431199
15. High Slit2 expression is associated with glioma. PMID: 27916173
16. ROBO1 mediated the inhibitory effect of miR-218 on angiogenesis in gastric cancer. PMID: 28323002
17. Results indicate the importance of SLIT2-ROBO1-CDC42 signaling pathway in predicting tumor progression. PMID: 27659325
18. We postulate that Robo1 promotes tumor invasion partly by the upregulation of MMP2 after activation of PI3K/Akt signaling pathway. Notably, Slit2 knockdown caused the upregulation of Robo1 expression both at the mRNA and protein levels. Thus, the stimulatory effects of Slit2 knockdown on tumor progression can be ascribed, at least in part, to the upregulation of Robo1 and its positive role in tumor progression. PMID: 27176045
19. ROBO1 deletion in a putative transcriptional regulatory region PMID: 26427657
20. Over-expression of miR-218 in glioma cells may inhibit the proliferation and tumorigenicity through targeting Robo1, suggesting that miR-218 could be a potential target for developing therapies in treating glioma. PMID: 26889813
21. Significantly increased serum Slit2 levels and hepatic expression of Slit2 and Robo1 were observed in patients with liver fibrosis. PMID: 26264936
22. expression pattern in extravillous trophoblasts associated with the remodeling events of the tubal pregnancy PMID: 26282852
23. our findings indicated that Slit2/Robo1 axis possibly be regarded as a significant clinical parameter for predicting brain metastasis in breast cancer patients. PMID: 26400100
24. miR-29a markedly inhibits the protein expression of Robo1 in mesenchymal stem cells. PMID: 26252416
25. A SLIT2/ROBO1 signaling circuit as a key regulatory mechanism. PMID: 26975850
26. These results suggest that Slit2/Robo1 binding exerts an effect on cell migration, which is negatively regulated by Robo4, and Robo1 may function by interacting with CdGAP in HUVECs. PMID: 26713366
27. ROBO1 somatic mutation is associated with myelodysplastic syndrome progression. Overexpression of ROBO1 produces anti-proliferative and pro-apoptotic effects in leukaemia cells. However, this effect was lost in ROBO mutants. PMID: 26608094
28. Robo1 promoted cell division cycle 42 (Cdc42) expression in HUVECs, and a distorted actin cytoskeleton in HUVECs was observed when Robo1 expression was suppressed. In conclusion, Robo1 promoted angiogenesis in HCC mediated by Cdc42. PMID: 26022159
29. these studies demonstrate that miR-219-5p inhibited cancer cell growth and invasion by direct targeting ROBO1, implicating miR-219-5p as an attractive candidate for cancer therapy. PMID: 26081620
30. Slit2/Robo1 signaling promotes intestinal tumorigenesis through Src-mediated activation of the Wnt/beta-catenin pathway. PMID: 25605242
31. miR29a inhibits cell migration and invasion in breast cancer cells, at least in part, by directly targeting Robo1. PMID: 25955714
32. In this review, we summarize recent findings demonstrating that the neuronal guidance cues, Slit and Roundabout (Robo), prevent the migration of multiple leukocyte subsets towards diverse inflammatory chemoattractants. PMID: 24777535
33. Inactivation of SLIT2 and/or ROBO1 is one of the early events in development of dysplastic lesions of head and neck and has prognostic importance. PMID: 25465073
34. identify two separate binding sites for heparin interaction with Robo1: one binding site at the previously identified site for heparin dp8 and a second binding site at the N terminus of Robo1 that is disordered in the x-ray crystal structure PMID: 25752613
35. prognostic implications of SLIT and ROBO1 expression in gallbladder cancer PMID: 24777813
36. Data show that ubiquitin specific peptidase 33 (USP33) mediates nerve tissue proteins Slit-Robo signaling in lung cancer cell migration. PMID: 24981056
37. ROBO1 was a functional target of miRNA-218's downstream pathway involving in cell invasion and migration of pancreatic cancer. PMID: 25010661
38. ROBO1 contributs to the deficits in developmental dyslexia and its correlated phenotypes. PMID: 24430574
39. Family-based analysis shows association of SNPs in ROBO1 with reading diasbilities. PMID: 24612512
40. Lower expression of ROBO1 is associated with prostate cancer disease progression. PMID: 24752651
41. Full-length Robo1 is present almost exclusively as a dimer; parallel studies demonstrate the biological activity of Slit2 and its interaction with Robo1. PMID: 24673457
42. Frameshift mutations of ROBO1 and ROBO2 genes and alteration of ROBO2 expression in gastric and colorectal cancers suggest that both genes might play roles in the pathogenesis of both cancers. PMID: 24247621
43. Downregulation of miRNA-218 and upregulation of ROBO-1 were first demonstrated in pancreatic cancer. PMID: 23733161
44. Low Robo1 expression was associated with cell proliferation and migration in ICC and was one of the adverse prognostic factors in patients with these tumors. PMID: 23953227
45. No genetic association of ROBO1 with developmental dyslexia was found in Indian population. PMID: 23954868
46. Data indicate that slit2N alters the localization and binding of Robo1 to WASp and LSP1 in HIV-1-gp120-treated immature dendritic cells (iDCs). PMID: 23119100
47. breast cancer cell migration and invasion was promoted when miRNA- 218 was significantly down regulated, in the way of up-regulation of Robo1. PMID: 22898079
48. data suggests the importance of abrogation of SLIT2-ROBO1 and SLIT2-ROBO2 interactions in the initiation and progression of CACX and also for early diagnosis and prognosis of the disease PMID: 22719878
49. we report that the tumorigenic potential of breast cancer cells is determined by an interaction between the Robo1 receptor and its ligand Slit2 PMID: 22826604
50. Robo1 expression correlates negatively with invasive ductal carcinoma brain metastasis, and correlates positively with the age and prognosis of IDC patients. PMID: 21875486
顯示更多

收起更多
亞細胞定位：

Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass membrane protein.
蛋白家族：

Immunoglobulin superfamily, ROBO family
組織特異性：

Widely expressed, with exception of kidney.
數據庫鏈接：

HGNC: 10249

OMIM: 602430

KEGG: hsa:6091

STRING: 9606.ENSP00000420321

UniGene: Hs.744218