NSUN2
NSUN2，即NOP2/Sun域家族成員2，是一種關鍵的tRNA甲基轉移酶，別名MISU、SAKI等。它源自人類基因表達，負責催化RNA分子中的m5C甲基化修飾，這一過程對RNA穩定性、翻譯效率及蛋白質功能至關重要。NSUN2在細胞增殖、發育及分化中扮演核心角色，其異常表達與多種癌癥的發生、進展及不良預后緊密相關。因此，NSUN2不僅是生物學研究的重要對象，也是癌癥治療領域潛在的靶標。通過調控NSUN2的表達或功能，可為癌癥的診斷、治療及預后評估提供新的策略。
熱銷產品
Recombinant Human RNA cytosine C(5)-methyltransferase NSUN2 (NSUN2) (CSB-EP626626HU)
驗證數據
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
NSUN2 Antibody (CSB-PA626626LA01HU)
驗證數據
Western Blot
Positive WB detected in: HepG2 whole cell lysate, Rat liver tissue, Mouse spleen tissue
All lanes: NSUN2 antibody at 6.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87, 83, 60 kDa
Observed band size: 87 kDa
Immunofluorescence staining of Hela cells with CSB-PA626626LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA626626LA01HU diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
NSUN2 Antibodies
NSUN2 for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA016110GA01HU | NSUN2 Antibody | Human,Mouse,Rat | ELISA,WB,IHC,IF |
| CSB-PA626626LA01HU | NSUN2 Antibody | Human, Rat, Mouse | ELISA, WB, IHC, IF |
| CSB-PA626626LB01HU | NSUN2 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA626626LC01HU | NSUN2 Antibody, FITC conjugated | Human | |
| CSB-PA626626LD01HU | NSUN2 Antibody, Biotin conjugated | Human | ELISA |
NSUN2 Proteins
NSUN2 Proteins for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-EP626626HU | Recombinant Human RNA cytosine C(5)-methyltransferase NSUN2 (NSUN2) | E.coli |
