HNF4A

HNF4A，即肝細(xì)胞核因子4α，別名NR2A1、TCF14、MODY1。HNF4A是一種核受體超家族成員，屬于配體依賴性轉(zhuǎn)錄因子，主要分布在肝臟中，在肝臟的發(fā)育和肝細(xì)胞分化成熟過程中起重要調(diào)控作用。HNF4A在轉(zhuǎn)錄水平上調(diào)控肝臟特異基因的表達(dá)，促進(jìn)肝細(xì)胞的發(fā)育和分化，參與肝細(xì)胞極性的建立和維持，增強(qiáng)肝臟的合成、代謝及解毒功能。HNF4A通過抑制肝星狀細(xì)胞的活化、逆轉(zhuǎn)上皮間質(zhì)細(xì)胞轉(zhuǎn)化、抑制肝癌細(xì)胞增殖、侵襲和轉(zhuǎn)移等機(jī)制，參與肝纖維化、肝硬化和肝細(xì)胞癌等疾病的發(fā)生和變化過程。HNF4A的表達(dá)異常與多種肝臟疾病相關(guān)，包括肝纖維化、肝硬化和肝癌。

熱銷產(chǎn)品

Recombinant Human Hepatocyte nuclear factor 4-alpha (HNF4A) (CSB-EP010594HU)

驗(yàn)證數(shù)據(jù)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP010594HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) HNF4A.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

HNF4A Recombinant Monoclonal Antibody (CSB-RA571090A0HU)

驗(yàn)證數(shù)據(jù)

Western Blot
Positive WB detected in: HL-60 whole cell lysate, Rat Liver tissue lysate,
All lanes: HNF-4-alpha antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

IHC image of CSB-RA571090A0HU diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.75% DAB.

Immunofluorescence staining of MCF-7 with CSB-RA571090A0HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 526-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing HepG2 cells stained with CSB-RA571090A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

HNF4A Antibody

HNF4A Protein

HNF4A Antibodies

HNF4A for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA443636	Phospho-HNF4A (Ser304) Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA000743	Phospho-HNF4A (S313) Antibody	Human,Mouse,Rat	WB, IHC, IF, ELISA
CSB-PA002939	HNF4A Antibody	Human,Mouse,Rat	WB, IHC, ELISA
CSB-PA004803	HNF4A Antibody	Human,Mouse,Rat	WB, ELISA
CSB-PA010594LA01HU	HNF4A Antibody	Human, Mouse	ELISA, WB
CSB-PA010594LB01HU	HNF4A Antibody, HRP conjugated	Human	ELISA
CSB-PA010594LC01HU	HNF4A Antibody, FITC conjugated	Human
CSB-PA010594LD01HU	HNF4A Antibody, Biotin conjugated	Human	ELISA
CSB-PA727840	Acetyl-HNF4A (Lys106) Antibody	Human,Mouse,Rat	WB,ELISA
CSB-RA571090A0HU	HNF4A Recombinant Monoclonal Antibody	Human, Rat	ELISA, WB, IHC, IF, FC

HNF4A Proteins

HNF4A Proteins for Rattus norvegicus (Rat)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP010594RA CSB-EP010594RA CSB-BP010594RA CSB-MP010594RA CSB-EP010594RA-B	Recombinant Rat Hepatocyte nuclear factor 4-alpha (Hnf4a)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

HNF4A Proteins for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP010594HU CSB-BP010594HU CSB-MP010594HU CSB-EP010594HU-B	Recombinant Human Hepatocyte nuclear factor 4-alpha (HNF4A)	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP010594HU	Recombinant Human Hepatocyte nuclear factor 4-alpha (HNF4A)	E.coli

HNF4A Proteins for Mus musculus (Mouse)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP010594MO CSB-EP010594MO CSB-BP010594MO CSB-MP010594MO CSB-EP010594MO-B	Recombinant Mouse Hepatocyte nuclear factor 4-alpha (Hnf4a)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

HNF4A Proteins for Xenopus laevis (African clawed frog)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP835521XBE CSB-EP835521XBE CSB-BP835521XBE CSB-MP835521XBE CSB-EP835521XBE-B	Recombinant Xenopus laevis Hepatocyte nuclear factor 4-alpha (hnf4a)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli