ENPP3
外核苷酸焦磷酸酶/磷酸二酯酶家族成員3是一種由ENPP3基因編碼的酶。Hiroaki在2013年的實(shí)驗中對N2a細(xì)胞進(jìn)行研究，純化了存在于GnT-IX中的一種內(nèi)源性抑制因子，發(fā)現(xiàn)其與外核苷酸焦磷酸酶/磷酸二酯酶3 (ENPP3)相同，這一結(jié)果通過質(zhì)譜分析以及培養(yǎng)細(xì)胞中ENPP3的敲除和過表達(dá)得到了證明。動力學(xué)分析顯示，ENPP3導(dǎo)致的GnT-IX抑制機(jī)制是ENPP3介導(dǎo)的核苷酸糖供體底物UDP-GlcNAc水解，從而生成UMP(一種強(qiáng)效競爭性GnT-IX抑制劑)。實(shí)際上，當(dāng)對細(xì)胞內(nèi)的ENPP3敲除，細(xì)胞內(nèi)核苷酸糖水平顯著升高，并顯示出細(xì)胞總糖基化特征的變化。除了糖基轉(zhuǎn)移酶的伴侶或其他已知調(diào)節(jié)劑外，ENPP3介導(dǎo)的核苷酸糖水解將對糖基轉(zhuǎn)移酶活性產(chǎn)生也會產(chǎn)生廣泛和顯著的影響，并負(fù)責(zé)改變細(xì)胞總糖基化特征和調(diào)節(jié)細(xì)胞功能。
熱銷產(chǎn)品
驗證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis ENPP3 at 2 μg/ml can bind Anti-ENPP3 recombinant antibody (CSB-RA007681MA1HU), the EC50 is 3.313-4.724 ng/mL.
驗證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ENPP3 at 2 μg/ml can bind anti-ENPP3 recombinant antibody (CSB-RA007681MA1HU), the EC50 is 2.151-2.492 ng/mL.
● ENPP3 Recombinant Monoclonal Antibody CSB-RA007681MA1HU
驗證數(shù)據(jù)
The Binding Activity of Macaca fascicularis ENPP3 with Anti-ENPP3 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis ENPP3 (CSB-MP4278MOV) at 2 μg/mL can bind Anti-ENPP3 recombinant antibody, the EC50 is 3.313-4.724 ng/mL.
The Binding Activity of Huamn ENPP3 with Anti- ENPP3 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ENPP3(CSB-MP007681HU) at 2 μg/mL can bind Anti-ENPP3 recombinant antibody, the EC50 is 2.151-2.492 ng/mL
Untransfected HEK293T cells (green line) and transfected Human ENPP3 HEK293T stable cells (red line) were stained with anti-ENPP3 recombinant antibody (2μg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
● HEK293T/Human ENPP3 Stable Cell Line CSB-SC007681HU
驗證數(shù)據(jù)
Untransfected HEK293T cells (green line) and transfected Human ENPP3 HEK293T Stable cells (red line) were stained with anti-ENPP3 antibody (2μg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
ENPP3 Antibodies
ENPP3 for Homo sapiens (Human)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA005288 | ENPP3 Antibody | Human,Mouse,Rat | WB, ELISA |
| CSB-PA575380 | ENPP3 Antibody | Human,Mouse,Rat | ELISA,IHC |
| CSB-PA448237 | ENPP3 Antibody | Human,Mouse,Rat | ELISA,IHC |
| CSB-RA007681MA1HU | ENPP3 Recombinant Monoclonal Antibody | Human, Macaca fascicularis | ELISA,FC |
ENPP3 Proteins
ENPP3 Proteins for Rattus norvegicus (Rat)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP007681RA CSB-EP007681RA CSB-BP007681RA CSB-MP007681RA CSB-EP007681RA-B |
Recombinant Rat Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (Enpp3), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ENPP3 Proteins for Bos taurus (Bovine)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP007681BO CSB-EP007681BO CSB-BP007681BO CSB-MP007681BO CSB-EP007681BO-B |
Recombinant Bovine Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (ENPP3), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ENPP3 Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP007681HU CSB-EP007681HU CSB-BP007681HU CSB-EP007681HU-B |
Recombinant Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (ENPP3), partial | Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli |
| CSB-MP007681HU | Recombinant Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (ENPP3), partial (Active) | Mammalian cell |
| CSB-MP007681HUb0 | Recombinant Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (ENPP3), partial (Active) | Mammalian cell |
ENPP3 Proteins for Pongo abelii (Sumatran orangutan)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP719125PYX CSB-EP719125PYX CSB-BP719125PYX CSB-MP719125PYX CSB-EP719125PYX-B |
Recombinant Pongo abelii Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (ENPP3), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ENPP3 Proteins for Mus musculus (Mouse)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP735351MO CSB-EP735351MO CSB-BP735351MO CSB-MP735351MO CSB-EP735351MO-B |
Recombinant Mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (Enpp3), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ENPP3 ELISA Kit
ENPP3 ELISA Kit for Homo sapiens (Human)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-EL007681HU | Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 3(ENPP3) ELISA kit | serum, plasma, tissue homogenates, cell lysates | 0.039 ng/mL |
