CSB-MP005508HU(A4) is detected by Mouse anti-6*His monoclonal antibody.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 at 10 μg/ml can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU), the EC₅₀ is 1.501-2.035 ng/mL

The presence of VLP-like structures was confirmed by TEM

Human CLDN6 Monoclonal Antibody (CSB-RA005508MA1HU) captured on Protein A Chip can bind Human CLDN6 Full Length VLP Protein with an affinity constant of 6.58 nM as detected by MetaSPR Assay (WeSPR^TM 200).

The purity of VLPs was greater than 95% as determined by SEC-HPLC

The Binding Activity of Huamn CLDN6 with Anti-CLDN6/9 recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 (CSB-MP005508HU(A4)) at 10 μg/mL can bind Anti-CLDN6/9 recombinant antibody, the EC₅₀ is 1.501-2.035 ng/mL.

The Binding Activity of Human CLDN9 with Anti-CLDN6/9 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 (CSB-MP005511HU) at 10 μg/mL can bind Anti-CLDN6/9 recombinant antibody, the EC₅₀ is 24.93-35.47 ng/mL.

Untransfected HEK293T cells surface (green line) and transfected Human CLDN6 HEK293T stable cells surface (red line) were stained with anti-CLDN6/9 recombinant antibody (2μg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Untransfected MC38 cells (green line) and transfected Human CLDN9 MC38 stable cells (red line) were stained with anti-CLDN6/9 antibody (2μg/1*10⁶cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Untransfected CHO-K1*10⁶cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Untransfected MC38 cells (green line) and transfected Human CLDN6 MC38 stable cells (red line) were stained with anti-CLDN6/9 antibody (2μg/1*10⁶cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

The Binding Activity of Macaca fascicularis CLDN6 with Anti-CLDN6/9 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CLDN6(CSB-MP6148GKN) at 5 μg/mL can bind Anti-CLDN6/9 recombinant antibody. The EC₅₀ is 4.186-6.635 ng/mL.
The VLPs (CSB-MP3838) is negative control.

The Binding Activity of Human CLDN6 with Anti-CLDN6/9 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 (CSB-MP005508HU(A5)-D) at 2 μg/mL can bind Anti-CLDN6/9 recombinant antibody. The EC₅₀ is 1.243-2.426 ng/mL.

The Binding Activity of Human CLDN9 with Anti-CLDN6/9 Recombinant Antibody
Activity：Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 (CSB-MP005511HU-D) at 2 μg/ml on an Nickel Coated plate can bind Anti-CLDN6/9 recombinant antibody. The EC₅₀?is 2.812-3.524 ng/mL.

The Binding Activity of Mouse cldn6 with Anti-CLDN6/9 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse cldn6(CSB-MP3347MO) at 5 μg/mL can bind Anti-CLDN6/9 recombinant antibody. The EC₅₀ is 4.027-6.270 ng/mL.

The Binding Activity of Human CLDN6 with Anti-CLDN6 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 (CSB-MP005508HU(A4)) at 10 μg/mL can bind Anti-CLDN6 recombinant antibody. The EC₅₀ is 2.033-2.415 ng/mL. The VLPs (CSB-MP3838) is negative control.

Untransfected HEK-293T cells (green line) and transfected Human CLDN6 HEK-293T stable cells (red line) were stained with anti-CLDN6 antibody (2μg/1*10⁶cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Untransfected MC38 cells (green line) and transfected Human CLDN6 MC38 stable cells (red line) were stained with anti-CLDN6 antibody (2μg/1*10⁶cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.