CD47
CD47，白細胞表面抗原 CD47，也被稱為整合素相關(guān)蛋白（IAP）或MER6蛋白。CD47屬于免疫球蛋白超家族成員，廣泛表達于多種細胞表面，由人類CD47基因編碼。CD47的作用機制主要涉及與信號調(diào)節(jié)蛋白α的相互作用，通過磷酸化激活SIRPα，招募和激活SHP磷酸酶，進而介導(dǎo)肌球蛋白2a去磷酸化，導(dǎo)致巨噬細胞對攜帶CD47的細胞的吞噬作用減弱，從而負調(diào)節(jié)吞噬作用。CD47在維持良性細胞的免疫耐受中起著關(guān)鍵作用，確保自體細胞不會被不適當(dāng)?shù)赝淌伞Ｈ欢S多不同類型的癌癥細胞利用CD47的高表達來介導(dǎo)免疫逃逸，逃避被巨噬細胞吞噬的命運。CD47的阻斷劑正在被開發(fā)用于促進巨噬細胞吞噬腫瘤細胞，作為癌癥治療的潛在策略。
熱銷產(chǎn)品
Recombinant Human Leukocyte surface antigen CD47 (CD47), partial (Active) (CSB-MP004940HU)
驗證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized SIRPA (CSB-MP021334HU) at 2 μg/ml can bind human CD47, the EC50 of human CD47 protein is 65.91-82.42 ng/ml.
Human SIRPA protein His/Myc tag (CSB-MP021334HU) captured on COOH chip can bind Human CD47 protein Fc tag (CSB-MP004940HU) with an affinity constant of 19.1 nM as detected by LSPR Assay.
CD47 (Magrolimab) Recombinant Monoclonal Antibody (CSB-RA004940MA1HU)
驗證數(shù)據(jù)
The Binding Activity of Human CD47 with Anti-CD47 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD47 (CSB-MP004940HUd7) at 2 μg/mL can bind Anti-CD47 recombinant antibody. The EC50 is 1.343-1.561 ng/mL.
Untransfected CT26 cells (green line) and transfected Human DLL3 & CD47 CT26 stable cells (red line) were stained with anti-CD47 antibody (CSB-RA004940MA1HU) (2μg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
Untransfected CT26 cells (green line) and transfected Human DLL3 & CD47 CT26 stable cells (red line) were stained with anti-DLL3 antibody (CSB-RA882142MA2HU) (2μg/1*106cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
CD47 Antibodies
CD47 for Homo sapiens (Human)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA004940GA01HU | CD47 Antibody | Human | ELISA,WB |
| CSB-PA005993 | CD47 Antibody | Human | WB, ELISA |
| CSB-PA445679 | CD47 Antibody | Human | ELISA,WB,IHC |
| CSB-PA911004 | CD47 Antibody | Human | ELISA,IHC |
| CSB-PA004940LA01HU | CD47 Antibody | Human | ELISA, IHC, IF |
| CSB-PA004940LB01HU | CD47 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA004940LC01HU | CD47 Antibody, FITC conjugated | Human | |
| CSB-PA004940LD01HU | CD47 Antibody, Biotin conjugated | Human | ELISA |
| CSB-RA802124A0HU | CD47 Recombinant Monoclonal Antibody | Human | ELISA, WB, IHC, IF |
| CSB-RA004940MA1HU | CD47 (Magrolimab) Recombinant Monoclonal Antibody | Human | ELISA, FC |
CD47 Proteins
CD47 Proteins for Rattus norvegicus (Rat)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP004940RA1 CSB-EP004940RA1 CSB-BP004940RA1 CSB-MP004940RA1 CSB-EP004940RA1-B |
Recombinant Rat Leukocyte surface antigen CD47 (Cd47), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF004940RA | Recombinant Rat Leukocyte surface antigen CD47 (Cd47) | in vitro E.coli expression system |
CD47 Proteins for Mus musculus (Mouse)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP713985MO1 CSB-EP713985MO1 CSB-BP713985MO1 CSB-MP713985MO1 CSB-EP713985MO1-B |
Recombinant Mouse Leukocyte surface antigen CD47 (Cd47), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF713985MO | Recombinant Mouse Leukocyte surface antigen CD47 (Cd47) | in vitro E.coli expression system |
| CSB-AP005351MO | Recombinant Mouse Leukocyte surface antigen CD47 (Cd47), partial (Active) | Mammalian cell |
CD47 Proteins for Pongo abelii (Sumatran orangutan)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP729089PYX1 CSB-EP729089PYX1 CSB-BP729089PYX1 CSB-MP729089PYX1 CSB-EP729089PYX1-B |
Recombinant Pongo abelii Leukocyte surface antigen CD47 (CD47), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF729089PYX | Recombinant Pongo abelii Leukocyte surface antigen CD47 (CD47) | in vitro E.coli expression system |
CD47 Proteins for Sus scrofa (Pig)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP872379PI1 CSB-EP872379PI1 CSB-BP872379PI1 CSB-MP872379PI1 CSB-EP872379PI1-B |
Recombinant Pig Leukocyte surface antigen CD47 (CD47), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF872379PI | Recombinant Pig Leukocyte surface antigen CD47 (CD47) | in vitro E.coli expression system |
CD47 Proteins for Bos taurus (Bovine)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP885628BO1 CSB-EP885628BO1 CSB-BP885628BO1 CSB-MP885628BO1 CSB-EP885628BO1-B |
Recombinant Bovine Leukocyte surface antigen CD47 (CD47), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF885628BO | Recombinant Bovine Leukocyte surface antigen CD47 (CD47) | in vitro E.coli expression system |
CD47 Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-AP005201HU | Recombinant Human Leukocyte surface antigen CD47 (CD47), partial (Active) | Mammalian cell |
| CSB-AP005211HU | Recombinant Human Leukocyte surface antigen CD47 (CD47), partial (Active) | Mammalian cell |
| CSB-MP004940HU | Recombinant Human Leukocyte surface antigen CD47 (CD47), partial (Active) | Mammalian cell |
| CSB-MP004940HUj8-B | Recombinant Human Leukocyte surface antigen CD47 (CD47), partial,Biotinylated | Mammalian cell |
| CSB-MP004940HU-B | Recombinant Human Leukocyte surface antigen CD47 (CD47), partial, Biotinylated | Mammalian cell |
| CSB-MP004940HUd7 | Recombinant Human Leukocyte surface antigen CD47 (CD47), partial (Active) | Mammalian cell |
CD47 ELISA Kit
CD47 ELISA Kit for Homo sapiens (Human)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-EL004940HU | Human Leukocyte surface antigen CD47(CD47) ELISA kit | serum, plasma, urine, tissue homogenates | 0.78 pg/mL |
CD47 ELISA Kit for Mus musculus (Mouse)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-EL004940MO | Mouse Leukocyte surface antigen CD47(CD47) ELISA kit | serum, plasma,tissue homogenates | 3.9 pg/mL |
