重組蛋白

重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實(shí)驗(yàn)室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項(xiàng)目中生成的蛋白質(zhì)。它們在許多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗(yàn)證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來支持您的研究,您來對地方了。
經(jīng)過18年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達(dá)重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達(dá)和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達(dá)技術(shù),以及膜蛋白表達(dá)技術(shù)等。
產(chǎn)品特點(diǎn)
- 超過13,000種重組蛋白覆蓋大多數(shù)基因/靶點(diǎn);
- 5種表達(dá)系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲細(xì)胞、哺乳動物細(xì)胞和體外大腸桿菌。而體外大腸桿菌表達(dá)系統(tǒng)是華美生物的獨(dú)特表達(dá)系統(tǒng)。與傳統(tǒng)蛋白表達(dá)系統(tǒng)相比,體外大腸桿菌省略了許多過程,如質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標(biāo)簽,包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用,包括抗原、細(xì)胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細(xì)胞培養(yǎng)-無血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標(biāo)準(zhǔn)及其原料、體內(nèi)研究、質(zhì)譜標(biāo)準(zhǔn)、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動物副產(chǎn)品的動物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請查看華美生物蛋白質(zhì)定制服務(wù)。
通過選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號 | 規(guī)格 |
---|---|---|
Recombinant Pelotomaculum thermopropionicum Gamma-glutamyl phosphate reductase (proA) | CSB-YP398877PYG CSB-EP398877PYG CSB-BP398877PYG CSB-MP398877PYG CSB-EP398877PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase | CSB-YP398878PYG CSB-EP398878PYG CSB-BP398878PYG CSB-MP398878PYG CSB-EP398878PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum UDP-N-acetylmuramate--L-alanine ligase (murC) | CSB-YP398879PYG CSB-EP398879PYG CSB-BP398879PYG CSB-MP398879PYG CSB-EP398879PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum Orotate phosphoribosyltransferase (pyrE) | CSB-YP398880PYG CSB-EP398880PYG CSB-BP398880PYG CSB-MP398880PYG CSB-EP398880PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum UPF0102 protein PTH_1707 (PTH_1707) | CSB-YP398881PYG CSB-EP398881PYG CSB-BP398881PYG CSB-MP398881PYG CSB-EP398881PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum ATP-dependent helicase/nuclease subunit A (addA), partial | CSB-YP398882PYG CSB-EP398882PYG CSB-BP398882PYG CSB-MP398882PYG CSB-EP398882PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum Anthranilate phosphoribosyltransferase (trpD) | CSB-YP398883PYG CSB-EP398883PYG CSB-BP398883PYG CSB-MP398883PYG CSB-EP398883PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum UPF0182 protein PTH_1387 (PTH_1387), partial | CSB-YP398884PYG CSB-EP398884PYG CSB-BP398884PYG CSB-MP398884PYG CSB-EP398884PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum Ribosomal protein S12 methylthiotransferase RimO (rimO) | CSB-YP398885PYG CSB-EP398885PYG CSB-BP398885PYG CSB-MP398885PYG CSB-EP398885PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum 30S ribosomal protein S2 (rpsB) | CSB-YP398886PYG CSB-EP398886PYG CSB-BP398886PYG CSB-MP398886PYG CSB-EP398886PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum Ribosome maturation factor RimP (rimP) | CSB-YP398887PYG CSB-EP398887PYG CSB-BP398887PYG CSB-MP398887PYG CSB-EP398887PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial | CSB-YP398888PYG CSB-EP398888PYG CSB-BP398888PYG CSB-MP398888PYG CSB-EP398888PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum UPF0473 protein PTH_1066 (PTH_1066) | CSB-YP398889PYG CSB-EP398889PYG CSB-BP398889PYG CSB-MP398889PYG CSB-EP398889PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum Cobyric acid synthase (cobQ), partial | CSB-YP398890PYG CSB-EP398890PYG CSB-BP398890PYG CSB-MP398890PYG CSB-EP398890PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L27 (rpmA) | CSB-YP398891PYG CSB-EP398891PYG CSB-BP398891PYG CSB-MP398891PYG CSB-EP398891PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum ATP-dependent Clp protease proteolytic subunit (clpP) | CSB-YP398892PYG CSB-EP398892PYG CSB-BP398892PYG CSB-MP398892PYG CSB-EP398892PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum Biotin synthase (bioB) | CSB-YP398893PYG CSB-EP398893PYG CSB-BP398893PYG CSB-MP398893PYG CSB-EP398893PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum 4-hydroxy-2-oxovalerate aldolase (PTH_0483) | CSB-YP398894PYG CSB-EP398894PYG CSB-BP398894PYG CSB-MP398894PYG CSB-EP398894PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L17 (rplQ) | CSB-YP398895PYG CSB-EP398895PYG CSB-BP398895PYG CSB-MP398895PYG CSB-EP398895PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L6 (rplF) | CSB-YP398896PYG CSB-EP398896PYG CSB-BP398896PYG CSB-MP398896PYG CSB-EP398896PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達(dá)載體?
表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。
根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.yidonglou.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022