重組蛋白

重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實驗室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項目中生成的蛋白質(zhì)。它們在許多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來支持您的研究,您來對地方了。
經(jīng)過18年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達技術(shù),以及膜蛋白表達技術(shù)等。
產(chǎn)品特點
- 超過13,000種重組蛋白覆蓋大多數(shù)基因/靶點;
- 5種表達系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲細胞、哺乳動物細胞和體外大腸桿菌。而體外大腸桿菌表達系統(tǒng)是華美生物的獨特表達系統(tǒng)。與傳統(tǒng)蛋白表達系統(tǒng)相比,體外大腸桿菌省略了許多過程,如質(zhì)粒轉(zhuǎn)化、細胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標簽,包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用,包括抗原、細胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細胞培養(yǎng)-無血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標準及其原料、體內(nèi)研究、質(zhì)譜標準、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動物副產(chǎn)品的動物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請查看華美生物蛋白質(zhì)定制服務(wù)。
通過選擇目標名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號 | 規(guī)格 |
---|---|---|
Recombinant Pongo abelii Dual specificity protein phosphatase 3 (DUSP3) | CSB-YP735818PYX CSB-EP735818PYX CSB-BP735818PYX CSB-MP735818PYX CSB-EP735818PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Mediator of RNA polymerase II transcription subunit 6 (MED6) | CSB-YP735819PYX CSB-EP735819PYX CSB-BP735819PYX CSB-MP735819PYX CSB-EP735819PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii General transcription factor 3C polypeptide 2 (GTF3C2), partial | CSB-YP735820PYX CSB-EP735820PYX CSB-BP735820PYX CSB-MP735820PYX CSB-EP735820PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii CDC42 small effector protein 1 (CDC42SE1) | CSB-YP735821PYX CSB-EP735821PYX CSB-BP735821PYX CSB-MP735821PYX CSB-EP735821PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Centrosomal protein of 70 kDa (CEP70), partial | CSB-YP735822PYX CSB-EP735822PYX CSB-BP735822PYX CSB-MP735822PYX CSB-EP735822PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Juxtaposed with another zinc finger protein 1 (JAZF1) | CSB-YP735823PYX CSB-EP735823PYX CSB-BP735823PYX CSB-MP735823PYX CSB-EP735823PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Vesicular, overexpressed in cancer, prosurvival protein 1 (VOPP1), partial | CSB-YP735824PYX CSB-EP735824PYX CSB-BP735824PYX CSB-MP735824PYX CSB-EP735824PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii S1 RNA-binding domain-containing protein 1 (SRBD1), partial | CSB-YP735825PYX CSB-EP735825PYX CSB-BP735825PYX CSB-MP735825PYX CSB-EP735825PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii 40S ribosomal protein S15 (RPS15) | CSB-YP735826PYX CSB-EP735826PYX CSB-BP735826PYX CSB-MP735826PYX CSB-EP735826PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1 (LINGO1), partial | CSB-YP735827PYX CSB-EP735827PYX CSB-BP735827PYX CSB-MP735827PYX CSB-EP735827PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii SH3-containing GRB2-like protein 3-interacting protein 1 (SGIP1), partial | CSB-YP735828PYX CSB-EP735828PYX CSB-BP735828PYX CSB-MP735828PYX CSB-EP735828PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Ras-related protein Rap-1b (RAP1B) | CSB-YP735829PYX CSB-EP735829PYX CSB-BP735829PYX CSB-MP735829PYX CSB-EP735829PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Probable leucine--tRNA ligase, mitochondrial (LARS2) | CSB-YP735830PYX CSB-EP735830PYX CSB-BP735830PYX CSB-MP735830PYX CSB-EP735830PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii UPF0488 protein C8orf33 homolog | CSB-YP735831PYX CSB-EP735831PYX CSB-BP735831PYX CSB-MP735831PYX CSB-EP735831PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Neuropathy target esterase (PNPLA6), partial | CSB-YP735832PYX CSB-EP735832PYX CSB-BP735832PYX CSB-MP735832PYX CSB-EP735832PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Histone-lysine N-methyltransferase EZH1 (EZH1), partial | CSB-YP735833PYX CSB-EP735833PYX CSB-BP735833PYX CSB-MP735833PYX CSB-EP735833PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Enhancer of mRNA-decapping protein 3 (EDC3), partial | CSB-YP735834PYX CSB-EP735834PYX CSB-BP735834PYX CSB-MP735834PYX CSB-EP735834PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Vacuolar ATPase assembly integral membrane protein VMA21 (VMA21), partial | CSB-YP735835PYX1 CSB-EP735835PYX1 CSB-BP735835PYX1 CSB-MP735835PYX1 CSB-EP735835PYX1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii 55 kDa erythrocyte membrane protein (MPP1) | CSB-YP735836PYX CSB-EP735836PYX CSB-BP735836PYX CSB-MP735836PYX CSB-EP735836PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Pongo abelii Kelch repeat and BTB domain-containing protein 2 (KBTBD2), partial | CSB-YP735837PYX CSB-EP735837PYX CSB-BP735837PYX CSB-MP735837PYX CSB-EP735837PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達載體?
表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。
根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.yidonglou.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022