Recombinant Rhizobium meliloti Sensor protein fixL (fixL)

in vitro E.coli expression system

中文名稱：

黑根瘤菌fixL重組蛋白
貨號：

CSB-CF320810RKU
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

fixL
Uniprot No.：

P10955
別名：

fixL; RA0670; SMa1229; Sensor protein FixL
種屬：

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
蛋白長度：

full length protein
表達區域：

1-505
氨基酸序列

MLSKSGIERTQWGRRVVRWRGDGVAAYIVAAIVTSSVLAIRMIRAEPIGEGLLLFSFIPA ILVVALIGGRNPILFAAGLSLVAAVSHQQISSADGPSVVELLVFGSAVLLIVALGEVLEA ARRAIDRTEDVVRARDAHLRSILDTVPDATVVSATDGTIVSFNAAAVRQFGYAEEEVIGQ NLRILMPEPYRHEHDGYLQRYMATGEKRIIGIDRVVSGQRKDGSTFPMKLAVGEMRSGGE RFFTGFIRDLTEREESAARLEQIQAELARLARLNEMGEMASTLAHELNQPLSAIANYSHG CTRLLRDMDDAVATRIREALEEVASQSLRAGQIIKHLREFVTKGETEKAPEDIRKLVEES AALALVGSREQGVRTVFEYLPGAEMVLVDRIQVQQVLINLMRNAIEAMRHVDRRELTIRT MPADPGEVAVVVEDTGGGIPEEVAGQLFKPFVTTKASGMGIGLSISKRIVEAHGGEMTVS KNEAGGATFRFTLPAYLDERIVAND
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Putative oxygen sensor; modulates the activity of FixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates FixJ.
基因功能參考文獻：
1. Time-resolved resonance Raman spectra of Sinorhizobium meliloti FixL upon oxygen dissociation, as well as upon carbon monoxide dissociation, show that there are three steps in the dynamic structural changes that result from ligand dissociation. PMID: 17469799
亞細胞定位：

Cell inner membrane; Multi-pass membrane protein.
數據庫鏈接：

KEGG: sme:SMa1229