Recombinant Rat Synaptotagmin-4 (Syt4)

in vitro E.coli expression system

中文名稱：

大鼠Syt4重組蛋白
貨號(hào)：

CSB-CF023040RA
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

in vitro E.coli expression system
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

基因名：

Syt4
Uniprot No.：

P50232
別名：

Syt4; Synaptotagmin-4; Synaptotagmin IV; SytIV
種屬：

Rattus norvegicus (Rat)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-425
氨基酸序列

MAPITTSRVEFDEIPTVVGIFSAFGLVFTVSLFAWICCQRRSAKSNKTPPYKFVHVLKGVDIYPENLSSKKKFGGDDKSEAKRKAALPNLSLHLDLEKRDLNGNFPKTNPKAGSSSDLENVTPKLFPETEKEAVSPESLKSSTSLTSEEKQEKLGTLFLSLEYNFEKKAFVVNIKEAQGLPAMDEQSMTSDPYIKMTILPEKKHKVKTRVLRKTLDPVFDETFTFYGVPYPHIQELSLHFTVLSFDRFSRDDVIGEVLVPLSGIELSDGKMLMTREIIKRNAKKSSGRGELLVSLCYQSTTNTLTVVVLKARHLPKSDVSGLSDPYVKVNLYHAKKRISKKKTHVKKCTPNAVFNELFVFDIPCESLEEISVEFLVLDSERGSRNEVIGRLVLGATAEGSGGGHWKEICDFPRRQIAKWHMLCDG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Synaptotagmin family member which does not bind Ca(2+). Involved in neuronal dense core vesicles (DCVs) mobility through its interaction with KIF1A. Upon increased neuronal activity, phosphorylation by MAPK8/JNK1 destabilizes the interaction with KIF1A and captures DCVs to synapses. Plays a role in dendrite formation by melanocytes.
基因功能參考文獻(xiàn)：
1. neuronal activity increased dense core vesicle capture via JNK-dependent phosphorylation of the S135 site of Synaptotagmin-4 PMID: 29166604
2. The increased expression of Synaptotagmin IV in optic nerve crush eyes displays the possible neuroprotective effect of bone marrow mononuclear cell treatment PMID: 22695963
3. Immunocytochemistry in rat chromaffin cells shows differential distribution of synaptotagmin-1, -4, -7, and -9. PMID: 21287204
4. These data support an inhibitory effect of syt IV on release of vesicles and their transmitter content PMID: 20735850
5. Rat and Drosophila synaptotagmin 4 have opposite effects during SNARE-catalyzed membrane fusion. PMID: 20688915
6. The effects of syt IV on fusion pores are discussed in terms of structural models and kinetic mechanisms. PMID: 20303854
7. present several lines of evidence showing that the Syt IV is normally localized in the Golgi or immature vesicles at the cell periphery and is sorted to fusion-competent mature dense-core vesicles in response to short nerve growth factor stimulation PMID: 12446703
8. NMR analysis of Ca2+ binding to synaptotagmin 4 PMID: 15311271
9. synaptotagmin IV protein trafficking from the Golgi to the cell periphery is regulated by forskolin PMID: 15496596
10. Cells with overexpressed rat SytIV were unaltered in basal release probability, Ca2+ dependence of release, short-term plasticity, & fusion pore kinetics. Mutant Syt I (less Ca2+ affinity) reduced release probability & altered Ca2+ dependence of release. PMID: 16407532
11. Downregulation of Syt IV reduced Ca(2+)-triggered exocytosis of dense core vesicles. Syt I silencing, however, had no effect on vesicular release. PMID: 18468511
12. Given the neuroendocrine functions of the posterior pituitary, changes in Syt IV levels could be involved in endocrine transitions involving alterations in the release of the neuropeptides oxytocin and vasopressin PMID: 19136969
13. Expression of Syt4 increases 9-fold at night in the rat pineal gland; treatment with dibutyryl cyclic AMP increases expresssion. PMID: 19103603
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亞細(xì)胞定位：

Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle membrane; Single-pass membrane protein.
蛋白家族：

Synaptotagmin family
組織特異性：

Widely expressed. Expressed in the brain. Expressed in pituitary gland, cerebellum, cortex, hypothalamus and hippocampus.
數(shù)據(jù)庫(kù)鏈接：

KEGG: rno:64440

STRING: 10116.ENSRNOP00000023593

UniGene: Rn.126675