Recombinant Mouse Transient receptor potential cation channel subfamily V member 6 (Trpv6)

中文名稱：

Recombinant Mouse Transient receptor potential cation channel subfamily V member 6 (Trpv6)
貨號：

CSB-CF852782MO
說明書：

MSDS Datasheet
規格：

￥6240
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Trpv6
Uniprot No.：

Q91WD2
別名：

TrpV6;Calcium transport protein 1;CaT1;Epithelial calcium channel 2;ECaC2
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length
來源：

in vitro E.coli expression system
分子量：

88.9 kDa
表達區域：

1-767aa
氨基酸序列

MGPLQREDRPALGGANVAPGSSPVGVWHQPQPPKEPAFHPMGWSLPKEKGLILCLWNKFCRWFHRQESWAQSRDEQNLLQQKRIWESPLLLAAKENDVQALSKLLKFEGCEVHQRGAMGETALHIAALYDNLEAAMVLMEAAPELVFEPMTSELYEGQTALHIAVINQNVNLVRALLARGASVSARATGSVFHYRPHNLIYYGEHPLSFAACVGSEEIVRLLIEHGADIRAQDSLGNTVLHILILQPNKTFACQMYNLLLSYDGGDHLKSLELVPNNQGLTPFKLAGVEGNIVMFQHLMQKRKHIQWTYGPLTSTLYDLTEIDSSGDDQSLLELIVTTKKREARQILDQTPVKELVSLKWKRYGRPYFCVLGAIYVLYIICFTMCCVYRPLKPRITNRTNPRDNTLMQQKLLQEAYVTPKDDLRLVGELVSIVGAVIILLVEIPDIFRLGVTRFFGQTILGGPFHVIIITYAFMVLVTMVMRLTNVDGEVVPMSFALVLGWCNVMYFARGFQMLGPFTIMIQKMIFGDLMRFCWLMAVVILGFASAFYIIFQTEDPDELGHFYDYPMALFSTFELFLTIIDGPANYDVDLPFMYSVTYAAFAIIATLLMLNLLIAMMGDTHWRVAHERDELWRAQVVATTVMLERKLPRCLWPRSGICGREYGLGDRWFLRVEDRQDLNRQRIRRYAQAFQQQDGLYSEDLEKDSGEKLETARPFGAYLSFPTPSVSRSTSRSSTNWERLRQGALRKDLRGIINRGLEDGEGWEYQI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Calcium selective cation channel that mediates Ca(2+) uptake in various tissues, including the intestine. Important for normal Ca(2+) ion homeostasis in the body, including bone and skin. The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both a rapid Ca(2+)-dependent and a slower Ca(2+)-calmodulin-dependent mechanism; the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating.
基因功能參考文獻：
1. Study suggest that amyloid beta-protein increase the expression of TRPC6 via NF-kappaB in BV-2 microglia and promotes the production of COX-2, which induces hippocampus neuron damage. PMID: 28458019
2. Study showed that TRPV6-expressing elements seem to be discretely organized in the brain of mouse. TRPV6-immunoreactive arcuate neurons co-express estrogen receptor alpha and the ion channel protein expression in the mediobasal hypothalamus showed correlation with estrous cycle. PMID: 28039038
3. TRPV6-/- mice spontaneously developed osteoarthritis at a younger age and had more severe manifestations of OA than wild-type controls. TRPV6 could regulate certain chondrocyte functions, including ECM secretion, cell proliferation, and apoptosis. PMID: 28063212
4. TRPV6 plays an essential role in bone metabolism and is a critical regulator in osteoclasts differentiation and bone resorption. PMID: 24686448
5. CAT1 overexpression prevents obesity-induced hypertension by reducing the influence of the SNS on the maintenance of arterial pressure but not by buffering pressor responses to stress. PMID: 26186712
6. Adra2a is expressed in the mesenchyme of the mouse stomach primordium at E11.5. Fzd5 and Trpv6 are expressed in the epithelial layer of the stomach primordium after E11.5. PMID: 22266179
7. focus on TRPV6 gene polymorphisms, the start of TRPV6 translation at a non-AUG codon and the functions of TRPV6 in intestinal Ca(2+) uptake, sperm maturation, and male fertility. PMID: 25372600
8. these data indicate that obesity-induced down-regulation of CAT1 expression and subsequent reduced bioavailability of nitric oxide may contribute to the development of obesity-induced hypertension. PMID: 25041756
9. Fibroblasts from two long-lived mice mutants (Snell dwarf and PAPP-A knockout) expressed higher levels of CAT1 (a ATF4 target gene) during stress, suggesting a connection to longevity. PMID: 24691093
10. A non-AUG start codon is used in both human and mouse, extending the N-terminus of the protein by 40 amino acids. The increased translation of the smaller TRPV6 cDNA version may overestimate the in vivo situation where translation efficiency may represent an additional mechanism to tightly control the TRPV6-mediated Ca(2+) entry to prevent deleterious Ca(2+) overload. PMID: 23612980
11. Augmented endothelial l-arginine transport attenuated the prohypertensive effects of systemic and renal oxidative stress, suggesting that manipulation of endothelial CAT1 may provide a new therapeutic approach for the treatment of cardiovascular disease. PMID: 24428817
12. Trpv6 excision affects epididymal Ca(2+) handling and male fertility to the same extent as the introduction of the D541A pore mutation, arguing against residual functions of the TRPV6(D541A) pore mutant in epididymal epithelial cells PMID: 22427671
13. Data show that the transient receptor potential ion channel TRPV6 is expressed only at low levels in osteoblasts and plays little functional role in osteoblastic calcium uptake. PMID: 22163264
14. Data suggest that TRPV6 and other proteins involved in transcellular Ca(2+) transport are dynamically expressed in bone cells, while TRPV6 appears not crucial for bone metabolism and matrix mineralization. PMID: 21732366
15. appropriate Ca(2)+ absorption and a consequent TRPV6-mediated decrease in the extracellular Ca(2)+ concentration toward the distal segments of the epididymal duct are essential for the acquisition of basic functions and the survival of spermatozoa. PMID: 21540454
16. TRPV6 contributes to intestinal calcium transport when dietary calcium supply is limited and in this condition indirectly regulates bone formation and/or mineralization. PMID: 20399919
17. The mechanism of dietary protection by high dietary calcium in a mouse model of colonic hyperplasia is reported. PMID: 20508153
18. found in supporting cells of the organ of Corti, with weak labelling in outer and inner hair cells; expression patterns identical in old and young animals PMID: 19922080
19. to characterize ECaC2 in more detail and to compare its properties with those of ECaC1 to obtain a better understanding of transcellular Ca2+ transport in epithelia. PMID: 11744752
20. TRPV5 and TRPV6 are regulated by 17beta-E2 and 1,25(OH)2D3, whereas dietary Ca2+ is positively involved in the regulation of TRPV6 only. PMID: 12620887
21. The rapid induction of intestinal CaT1 mRNA expression by 1,25(OH)(2)D(3), and the marked induction at weaning, suggest that CaT1 is critical for 1,25(OH)(2)D(3)-mediated intestinal Ca absorption. PMID: 12933662
22. CaT1 upregulation causes vitamin D-independent effects at the genomic level on active duodenal calcium absorption mechanisms in hormonal changes during pregnancy or lactation PMID: 14584880
23. These results suggest that calmodulin positively affects transient receptor potential channel TRPV6 activity upon calcium binding to EF-hands 3 and 4, which involves the N and C termini and the transmembrane domain of TRPV6. PMID: 15123711
24. binding site for NHERF4 localized in a conserved region in the carboxyl terminus of TRPV5 and was distinct from the binding site of the PDZ protein NHERF2. NHERF4 predominantly localized at the plasma membrane of X. PMID: 16565876
25. downregulation of TRPV5 and TRPV6 is likely involved in the impaired Ca2+ (re)absorption during aging PMID: 16705151
26. Prednisolone reduces the intestinal Ca2+ absorption capacity through diminished duodenal expression of the active Ca2+ transporters TRPV6. PMID: 16901990
27. One aspect of vestibular dysfunction in Pendred syndrome is a pathological elevation of endolymphatic [Ca(2+)] due to luminal acidification and consequent inhibition of TRPV5/6-mediated Ca(2+) absorption. PMID: 17200157
28. For uterine function in normal pregnancy, TRPV6 is regulated by E2 via an ERalpha-dependent pathway. PMID: 17374692
29. PDZK2 is an essential TRPV6 interacting protein as a physiological modulator of TRPV6. PMID: 17645868
30. a novel TRPV6 regulatory mechanism which presumably participates in calcium homeostasis. PMID: 18005662
31. CaT1 may stimulate the intestinal absorption of Cd and Zn, and some Cd may be distributed to the kidneys along with MT-1 induced by Zn. PMID: 18054826
32. A different functional role for the TRPV6-ankyrin repeat domain than in the paralogous thermosensitive TRPV1 channel. PMID: 18232717
33. Study provides evidence using null mutant mice that significant active intestinal calcium transport occurs in the absence of TRPV6 and calbindin. PMID: 18325990
34. These results provide the first in vivo evidence that TRPV6 is involved in maternal-fetal Ca(2+) transport. PMID: 18348695
35. Ca(2+) influx through TRPV6 activates phospholipase C and the resulting depletion of PI(4,5)P(2) contributes to the inactivation of TRPV6. PMID: 18390907
36. TRPV6 is not required for vitamin D-induced intestinal calcium absorption and may not carry out a significant role in this process. PMID: 19073913
37. TRPV6 gene may function as a key element in controlling calcium transport in the uterus between the embryo and the placenta during pregnancy PMID: 19457270
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亞細胞定位：

Cell membrane; Multi-pass membrane protein.
蛋白家族：

Transient receptor (TC 1.A.4) family, TrpV subfamily, TRPV6 sub-subfamily
組織特異性：

Detected in intestine (at protein level). Abundantly expressed in pancreas and placenta, and to a much lesser extent in stomach and kidney. Detected in kidney and duodenum.
數據庫鏈接：

KEGG: mmu:64177

STRING: 10090.ENSMUSP00000031902

UniGene: Mm.296889