Recombinant Mouse Hepatic sodium/bile acid cotransporter (Slc10a1)

中文名稱：

Recombinant Mouse Hepatic sodium/bile acid cotransporter (Slc10a1)
貨號(hào)：

CSB-CF021373MO
說(shuō)明書：

MSDS Datasheet
規(guī)格：

￥9720
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Slc10a1
Uniprot No.：

O08705
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Full Length
來(lái)源：

in vitro E.coli expression system
分子量：

40.9 kDa
表達(dá)區(qū)域：

1-362aa
氨基酸序列

MEAHNVSAPFNFSLPPGFGHRATDTALSVILVVMLLLIMLSLGCTMEFSKIKAHFWKPKGVIIAIVAQYGIMPLSAFLLGKVFHLTSIEALAILICGCSPGGNLSNLFTLAMKGDMNLSIVMTTCSSFTALGMMPLLLYIYSKGIYDGDLKDKVPYKGIMLSLVMVLIPCAIGIFLKSKRPHYVPYVLKAGMIITFSLSVAVTVLSVINVGNSIMFVMTPHLLATSSLMPFTGFLMGYILSALFRLNPSCRRTISMETGFQNVQLCSTILNVTFPPEVIGPLFFFPLLYMIFQLAEGLLFIIIFRCYLKIKPQKDQTKITYKAAATEDATPAALEKGTHNGNNPPTQPGLSPNGLNSGQMAN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium.
基因功能參考文獻(xiàn)：
1. NTCP and OATP isoforms cooperatively form a stable bile acid uptake machinery in mice, whereas NTCP is predominant in humans. PMID: 28498614
2. Isoniazid/rifampicin administration significantly down-regulated the expression of hepatic bile acids transporters Ntcp and Bsep in liver. PMID: 25886055
3. Ntcp plasma membrane localization is regulated by hyperosmolarity and tauroursodeoxycholate PMID: 26306036
4. central role in hepatic uptake of conjugated bile acids and hepatitis B virus preS1/Myrcludex B binding PMID: 25641256
5. mNTCP was found to be unable to support either HBV or HDV infection, although it can bind to pre-S1 of HBV L protein and is functional in transporting substrate taurocholate. PMID: 23678176
6. hepatic transporters Ntcp and Mrp2 are downregulated in rodent models of necrotizing enterocolitis PMID: 23125159
7. Large variations in hepatic bile salt flux have minor effects on expression of murine Ntcp and Bsep in vivo, suggesting that these transporters are abundantly expressed and able to accommodate a wide range of 'physiological' bile salt fluxes. PMID: 12399219
8. Conserved NTCP/Ntcp 5'-regulatory region transcription regulation differs among species and is not directly regulated by small heterodimer partner. Bile acids may regulate NTCP/Ntcp indirectly by modulating nuclear factor regulation of gene expression. PMID: 14701722
9. Reduced levels of HNF-1alpha, RXRalpha, and RARalpha in CBDL FXR-/- mice and reduced DNA binding in CA-fed FXR-/- mice, despite unchanged Ntcp levels, indicate that these factors may have a minor role in regulation of mouse Ntcp during cholestasis PMID: 16002565
10. The C51A and C106A mutants of Slc10a2 showed significantly reduced TCA uptake, while no apparent difference in TCA uptake was observed for the Slc10a1-C44A mutant. PMID: 17690477
11. Ntcp and Bsep are regulated by age, gender, cholestyramine, and bile acid, but resistant to induction by most microsomal enzyme inducers. PMID: 17897632
12. mouse Ntcp is regulated by HNF-4alpha via a conserved distal cis-element independently of HNF-1alpha. PMID: 18483185
13. Report critical role of PPAR-alpha in perfluorooctanoic acid- and perfluorodecanoic acid-induced downregulation of Ntcp uptake transporter in mouse liver. PMID: 18703564
14. In the liver, histone H3 in the proximal promoter of Ntcp is hyperacetylated, whereas acetylation in the kidney and cerebrum is minimal. PMID: 19047482
15. Report Role of hepatic Ntcp transporter in prevention of bile acid toxicity after partial hepatectomy in mice. PMID: 19497955
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亞細(xì)胞定位：

Membrane; Multi-pass membrane protein.
蛋白家族：

Bile acid:sodium symporter (BASS) (TC 2.A.28) family
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:20493

STRING: 10090.ENSMUSP00000093229

UniGene: Mm.486361