Recombinant Mouse Orexin receptor type 2 (Hcrtr2)

in vitro E.coli expression system

中文名稱：

小鼠Hcrtr2重組蛋白
貨號：

CSB-CF010232MO
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

Hcrtr2
Uniprot No.：

P58308
別名：

Hcrtr2; Mox2r; Orexin receptor type 2; Ox-2-R; Ox2-R; Ox2R; Hypocretin receptor type 2
種屬：

Mus musculus (Mouse)
蛋白長度：

Full length protein
表達區域：

1-460
氨基酸序列

MSSTKLEDSLSRRNWSSASELNETQEPFLNPTDYDDEEFLRYLWREYLHPKEYEWVLIAG YIIVFVVALIGNVLVCVAVWKNHHMRTVTNYFIVNLSLADVLVTITCLPATLVVDITETW FFGQSLCKVIPYLQTVSVSVSVLTLSCIALDRWYAICHPLMFKSTAKRARNSIVVIWIVS CIIMIPQAIVMECSSMLPGLANKTTLFTVCDEHWGGEVYPKMYHICFFLVTYMAPLCLMI LAYLQIFRKLWCRQIPGTSSVVQRKWKQQQPVSQPRGSGQQSKARISAVAAEIKQIRARR KTARMLMVVLLVFAICYLPISILNVLKRVFGMFTHTEDRETVYAWFTFSHWLVYANSAAN PIIYNFLSGKFREEFKAAFSCCLGVHHRQGDRLARGRTSTESRKSLTTQISNFDNVSKLS EHVVLTSISTLPAANGAGPLQNWYLQQGVPSSLLSTWLEV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides. Triggers an increase in cytoplasmic Ca(2+) levels in response to orexin-A binding.
基因功能參考文獻：
1. In an heart failure model, HCRTR2-deficient mice exhibited poorer cardiac function, worse treadmill exercise capacity, and greater myocardial scarring PMID: 26653627
2. orexin activation of OX2R in the brain centrally enhances bone formation by lowing circulating leptin level. PMID: 24794976
3. Knocking down the Orx2 receptors in the basolateral amygdala increased anxious behavior as measured by reduced social preference and reduced time spent in the center of an open field. PMID: 24485472
4. OX2R activation induces PKC-mediated ERK and CREB phosphorylation. PMID: 22652455
5. In the hypothalamus, the OX2 receptors have an inhibitory role in modulating basal concentrations of NE, ACh, and Hist. In the prefrontal cortex, the evoked release of the monoamines NE, 5-HT, and DA seems to be controlled negatively by OX2 receptors. PMID: 22038504
6. Mice lacking OX2R signaling had poor maintenance of wakefulness indicative of sleepiness and fragmented sleep and lacked any electrophysiological response to orexin-A in the wake-promoting neurons of the tuberomammillary nucleus. PMID: 21368172
7. activation of orexin neurons through OX2R might have an important role in the maintenance of arousal. PMID: 20861370
8. Regulation of sleep/wake states is completely achieved by OX(2)R-expressing neurones without involving H(1)R-mediated pathways. Maintenance of basal physiological sleep/wake states is fully achieved without both H(1) and OX(1) receptors. PMID: 19694625
9. So, the SAF diet can alter adipocytic adiposity-related gene expression and result in effective amelioration of diet-induced obesity. PMID: 20213045
10. hypocretin receptor 2 mRNA levels declined with age in the hippocampus, thalamus, pons, and medulla (hypocretin receptor 2) PMID: 12399012
11. OX2alphaR is expressed in various areas of the mouse brain and has low expression in caudal part of cerebellum PMID: 16806113
12. The enhanced orexin-orexin receptor2 signaling confers resistance to diet-induced features of the metabolic syndrome through negative energy homeostasis and improved leptin sensitivity. PMID: 19117547
13. Biochemical and behavioural characterization of EMPA, a novel high-affinity, selective antagonist for the OX(2) receptor. PMID: 19751316
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亞細胞定位：

Cell membrane; Multi-pass membrane protein.
蛋白家族：

G-protein coupled receptor 1 family
組織特異性：

Widely expressed. Isoform 2 not detected in skeletal muscle and kidney.
數據庫鏈接：

KEGG: mmu:387285

STRING: 10090.ENSMUSP00000058230

UniGene: Mm.335300