Recombinant Human Sphingomyelin phosphodiesterase 2 (SMPD2)-VLPs

中文名稱：

Recombinant Human Sphingomyelin phosphodiesterase 2 (SMPD2)-VLPs
貨號：

CSB-MP021846HU
說明書：

MSDS Datasheet
規格：
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

SMPD2
Uniprot No.：

O60906
別名：

(Lyso-platelet-activating factor-phospholipase C)(Lyso-PAF-PLC)(Neutral sphingomyelinase)(N-SMase)(nSMase)(nSMase1)
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

Mammalian cell
表達區域：

1-423aa
氨基酸序列

MKPNFSLRLRIFNLNCWGIPYLSKHRADRMRRLGDFLNQESFDLALLEEVWSEQDFQYLRQKLSPTYPAAHHFRSGIIGSGLCVFSKHPIQELTQHIYTLNGYPYMIHHGDWFSGKAVGLLVLHLSGMVLNAYVTHLHAEYNRQKDIYLAHRVAQAWELAQFIHHTSKKADVVLLCGDLNMHPEDLGCCLLKEWTGLHDAYLETRDFKGSEEGNTMVPKNCYVSQQELKPFPFGVRIDYVLYKAVSGFYISCKSFETTTGFDPHRGTPLSDHEALMATLFVRHSPPQQNPSSTHGPAERSPLMCVLKEAWTELGLGMAQARWWATFASYVIGLGLLLLALLCVLAAGGGAGEAAILLWTPSVGLVLWAGAFYLFHVQEVNGLYRAQAELQHVLGRAREAQDLGPEPQPALLLGQQEGDRTKEQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 10xHis-tagged
If you have specified tag type, please tell us and we will check if it's possible to develop.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from PBS, 6% Trehalose, pH 7.4.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex. Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Hydrolyze 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor).
基因功能參考文獻：
1. Interestingly, neutral but not acid sphingomyelinase was mandatory for PorBIA -mediated Neisseria gonorrhoeae invasion into host cells. PMID: 25224994
2. nSMase1 (sphingomyelin phosphodiesterase 2, SMPD2)has a common central role in ceramide signaling during the stress and cytokine responses and apoptosis. PMID: 25168245
3. These data suggest that Ox-LDL activates N-SMase-ceramide signaling pathway, and stimulates phosphorylation of p38 MAPK, leading to apoptosis in vascular smooth muscle cells, which initiates VSMC calcification. PMID: 24358176
4. NSM2 plays a key role in the cellular response to hyperosmolar stress, and its activity regulates both cytokine secretion and lipid droplet formation. PMID: 22899568
5. nSMase2 has a role in ceramide generation, aberrant apoptosis, and lung injury under cigarette smoke exposure PMID: 20448054
6. N-SMase at the cytofacial plasma membrane is an essential element for the proper orientation of PMNs in FMLP gradients, at least in part by polarizing the distribution of Rac 1/2 and RhoA GTPases. PMID: 20378749
7. Results demonstrate that WithaD enhance the ceramide accumulation by activating N-SMase 2, modulate phosphorylation of the JNK and p38MAPK and induced apoptosis in both myeloid and lymphoid cells along with primary cells derived from leukemia patients. PMID: 20836852
8. Data show DA remarkably increased the NSMase2 message and protein, whereas little change in NSMase1 and NSMase3 mRNAs. PMID: 19698806
9. nSMase2 functions as a growth suppressor in MCF7 cells, linking confluence to the G(0)/G(1) cell cycle check point. PMID: 15051724
10. identification of 15 polymorphisms, including 9 novel SNPs, in the SMPD2 gene; one haplotype showed associations with the extrinsic type of atopic dermatitis subgroup PMID: 17212982
11. The catalytic region of neutral sphingomyelinase 2 where palmitoylation occurs is found to be localized at the inner leaflet of the plasma membrane. PMID: 17272284
12. This study underlines the importance of neutral sphingomyelinase-ceramide pathway in mediating oxidative stress-induced apoptosis and cell death of human primary oligodendrocytes. PMID: 18040843
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亞細胞定位：

Membrane; Multi-pass membrane protein.
蛋白家族：

Neutral sphingomyelinase family
數據庫鏈接：

HGNC: 11121

OMIM: 603498

KEGG: hsa:6610

STRING: 9606.ENSP00000258052

UniGene: Hs.55235