Recombinant Human Solute carrier family 22 member 3 (SLC22A3)

in vitro E.coli expression system

中文名稱：

人SLC22A3重組蛋白
貨號：

CSB-CF021458HU
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

SLC22A3
Uniprot No.：

O75751
別名：

SLC22A3; EMTH; OCT3; Solute carrier family 22 member 3; Extraneuronal monoamine transporter; EMT; Organic cation transporter 3
種屬：

Homo sapiens (Human)
蛋白長度：

Full length protein
表達區域：

1-556
氨基酸序列

MPSFDEALQRVGEFGRFQRRVFLLLCLTGVTFAFLFVGVVFLGTQPDHYWCRGPSAAALA ERCGWSPEEEWNRTAPASRGPEPPERRGRCQRYLLEAANDSASATSALSCADPLAAFPNR SAPLVPCRGGWRYAQAHSTIVSEFDLVCVNAWMLDLTQAILNLGFLTGAFTLGYAADRYG RIVIYLLSCLGVGVTGVVVAFAPNFPVFVIFRFLQGVFGKGTWMTCYVIVTEIVGSKQRR IVGIVIQMFFTLGIIILPGIAYFIPNWQGIQLAITLPSFLFLLYYWVVPESPRWLITRKK GDKALQILRRIAKCNGKYLSSNYSEITVTDEEVSNPSFLDLVRTPQMRKCTLILMFAWFT SAVVYQGLVMRLGIIGGNLYIDFFISGVVELPGALLILLTIERLGRRLPFAASNIVAGVA CLVTAFLPEGIAWLRTTVATLGRLGITMAFEIVYLVNSELYPTTLRNFGVSLCSGLCDFG GIIAPFLLFRLAAVWLELPLIIFGILASICGGLVMLLPETKGIALPETVDDVEKLGSPHS CKCGRNKKTPVSRSHL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Mediates potential-dependent transport of a variety of organic cations. May play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain.
基因功能參考文獻：
1. Data show that the common variation in the solute carrier family 22 member 3 (SLC22A3) gene is unlikely to significantly contribute to pancreatic cancer risk, and the rs2504938 single nucleotide polymorphism (SNP) in SLC22A3 significantly associates with an unfavorable prognosis of pancreatic cancer patients. PMID: 28272475
2. SNPs in SLC22A3 and H3F3B may influence lipid levels through altering the expression of local genes. PMID: 29894858
3. A-to-I RNA editing of SLC22A3 contributes to the early development and progression of familial esophageal squamous cell carcinoma in high-risk individuals. PMID: 28533408
4. our study suggests that several PHACTR1 and SLC22A3 gene polymorphisms may exert a protective effect against the CAD in the Chinese Han male population. PMID: 27893421
5. SLC22A3 deletion is associated with motor speech disorders, and language delays. PMID: 28767196
6. may be a regulator of the concentration of norepinephrine in adipose tissue. PMID: 28034777
7. the rs3088442G>A variant as a genetic marker may potentially assist in the identification of individuals at an increased risk of T2D. PMID: 28625319
8. The genotype of rs3088442 within the SLC22A3-LPAL2-LPA gene cluster may contribute to regulation of plasma Lp(a) levels and possibly to the severity of coronary artery disease in a Chinese Han population. PMID: 27417586
9. Findings suggest that OCT3 plays an important role in the absorption and elimination of metformin and that the transporter is a critical determinant of metformin bioavailability, clearance, and pharmacologic action. PMID: 25920679
10. The markers of EMT were detected by using Western blot. PMID: 25322669
11. Findings suggest a negative feedback mechanism against inflammatory response by which solute carrier family 22 member 3 (SLC22A3) variant rs3088442 G-->A decreased the risk of coronary heart disease (CHD). PMID: 25561729
12. Cultured astroctye line 1321N1 and primary human astrocytes transport monoamines partly through OCT3. PMID: 24471494
13. There was no association between rs7758229 in 6q26-q27/SLC22A3 and the risk of colorectal cancer in a Chinese population. PMID: 23555006
14. Decreasing expression of OCT3 and MATE1 in human placenta indicates these transporters may play a role in fetal protection preferentially at earlier stages of gestation. PMID: 23303678
15. Our studies demonstrate that genetic polymorphisms in the proximal promoter region of OCT3 alter the transcription rate of the gene and may be associated with altered expression levels of OCT3 in human liver. PMID: 22231567
16. The increased Wnt3 in the trastuzumab-resistant cells also promoted a partial EMT-like transition. PMID: 23071104
17. PDLIM5 (rs17021918,T), SLC22A3 (rs9364554,C) and NKX3-1 (rs1512268,A) SNPs might not be associated with prostate cancer in Chinese men. PMID: 22741436
18. The risk for coronary artery disease in a Chinese Han population is not associated with single nucleotide polymorphisms in the SLC22A3-LPAL2-LPA gene cluster. PMID: 23036009
19. NUDT11, HNF1B, and SLC22A3 genes have roles in prostate cancer pathogenesis PMID: 22730461
20. SLC22A3 was expressed high in the human heart with strongest OCT3 immunoreactivity in vascular endothelial cells. SLC22A3/OCT3 expression was not changed in failing human left ventricular myocardium PMID: 21697722
21. OCT3 overexpression significantly increased cisplatin cellular accumulation and cytotoxicity in KB-3-1 cells. PMID: 21905038
22. evidence for a new means of downregulating IL-4 production by basophils, both in vitro and in vivo, through OCT3 targeted by 5-HT and pharmacologic ligands. PMID: 21636115
23. Genetic association studies indicate 5 SNPs are associated with reduced transport activity of OCT3 (using 5-HT & MPP). PMID: 20562519
24. The regulation of EMT-mediated transport by second-messenger phosphorylation/dephosphorylation mechanisms has been characterized in stably transfected HEK293 cells with tritiated 1-methyl-4-phenylpyridinium as substrate. PMID: 11770002
25. EMT efficiently translocates agmatine and must be considered for the control of agmatine levels. PMID: 12538837
26. Genetic variation of EMT was studied in Caucasians. PMID: 12768439
27. EMT is expressed in the area postrema of rat brain and may play a role in physiological functions of this circumventricular organ such as emesis, food intake and the regulation of cardiovascular functions. PMID: 14690517
28. organic cation transporter EMT mRNA was mainly detected in the intra lobular septa and also expressed in scattered cells of placental vessel adventitias with lower expression of EMT mRNAs in pre-eclamptic placentae PMID: 15135235
29. Ranitidine and famotidine elicited differential inhibitory activities on SLC22A3. PMID: 16141367
30. These results suggest that polymorphisms of SLC22A3 are related to the development of polysubstance use in Japanese patients with methamphetamine (MAP) dependence. PMID: 17010131
31. PMAT, EMT, and OCT2 transporters are expressed in the endometrial stroma and can potentially regulate reuptake of monoamines in general and histamine in particular. PMID: 17393420
32. rare mutations in the EMT gene suggest a causative or modulating role in genetic subtypes of obsessive-compulsive disorder PMID: 17477885
33. Confirm the usefulness of Caki-1 cells as a proximal tubule model system for investigations of OCT3. PMID: 18253050
34. We conclude that the proliferation of immature germ cells in GB may be due to an interaction between OCT3/4 and accumulated beta-catenin in the nuclei of the immature germ cells. PMID: 18295396
35. Identification of the SLC22A3-LPAL2-LPA gene cluster as a strong susceptibility locus for coronary artery disease. PMID: 19198611
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亞細胞定位：

Membrane; Multi-pass membrane protein.
蛋白家族：

Major facilitator (TC 2.A.1) superfamily, Organic cation transporter (TC 2.A.1.19) family
組織特異性：

Expressed in placenta, skeletal muscle, prostate, aorta, liver, fetal lung, salivary gland, adrenal gland, kidney and brain cortex. No expression detected in spleen.
數據庫鏈接：

HGNC: 10967

OMIM: 604842

KEGG: hsa:6581

STRING: 9606.ENSP00000275300

UniGene: Hs.567337