Recombinant Bovine Lysosomal-associated transmembrane protein 4B (LAPTM4B)

in vitro E.coli expression system

中文名稱：

牛LAPTM4B重組蛋白
貨號：

CSB-CF765155BO
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

LAPTM4B
Uniprot No.：

Q71SV0
別名：

LAPTM4B; Lysosomal-associated transmembrane protein 4B
種屬：

Bos taurus (Bovine)
蛋白長度：

full length protein
表達區域：

1-226
氨基酸序列

MKMVAPWTRFYSNSCCLCCHVRTGTILLGVWYLILNAVVLLILLSALADPDHYHFSSSEL GGDFEFMDDANMCIAIAISVLMILICAMATYGAYKQRAAWIIPFFCYQIFDFALNTLVAV TVLVYPNSIQEYIRQLPPDFPYKDDIMSVNPTCLVLIILLFISIILAFKGYLISCVWNCY RYINGRNSSDVLVYVTSNDSTVLLPPYDDATVNSATKEPPPPYVSA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Required for optimal lysosomal function. Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation. Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation. Plays a role as negative regulator of TGFB1 production in regulatory T cells. Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways.
基因功能參考文獻：
1. This study reports for the first time that bovine LAPTM4B in granulosa cells is present in both unubiquinated and ubiquinated forms, and is differentially expressed in developing ovarian follicles PMID: 25881887
2. LAPTM4B is differentially expressed at the mRNA and protein levels in different bovine tissues. The mRNA yields a 26.3 kDa protein that undergoes mono-ubiquitination resulting in a 31.5 kDa isoform. Immunohistochemical observations revealed that LAPTM4B expression was highly variable among tissues, and among similar and adjacent cell types. LAPTM4B may confer protection against the accumulation of metabolic byproducts. PMID: 25881887
亞細胞定位：

Endomembrane system; Multi-pass membrane protein. Late endosome membrane. Cell membrane. Cell projection. Lysosome membrane. Endosome membrane. Endosome, multivesicular body membrane. Endosome, multivesicular body lumen.
蛋白家族：

LAPTM4/LAPTM5 transporter family
組織特異性：

Strongly expressed in fetal ovary, testis, adrenal gland, liver and uterus, and weakly expressed in the spleen.
數據庫鏈接：

KEGG: bta:404155

STRING: 9913.ENSBTAP00000022559

UniGene: Bt.1143