V5-Tag Monoclonal Antibody
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中文名稱：V5-Tag鼠單克隆抗體
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貨號：CSB-MA000161M1m
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規(guī)格：￥400
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圖片：
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Western Blot
Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4, 5, 6 at 50ng
All lanes: V5 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 31.9, 20.4, 55, 24.2, 53.4, 30 KDa
Observed band size: 32, 21, 55, 28, 58, 33 KDa
Exposure time: 5min -
Western Blot
Positive WB detected in: 50ng V5-tagged fusion protein
V5 Tag antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 32 KDa
Observed band size: 32 KDa
Exposure time: 5min -
Western Blot
Positive WB detected in: Mouse anti V5 tag Monoclonal antibody at 1:2000
Lane 1: Recombinant V5-tagged fusion protein at 25ng
Lane 2: Recombinant V5-tagged fusion protein at 12.5ng
Lane 3: Recombinant V5-tagged fusion protein at 6.25ng
Lane 4: Recombinant V5-tagged fusion protein at 3.125ng
Lane 5: Recombinant V5-tagged fusion protein at 1.56ng
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 32 kd
Observed band size: 32 kd
Exposure time: 5min -
Immunofluorescence staining of transfected HEK293F cells with CSB-MA000161M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor R-PE Goat Anti-Mouse IgG(H+L). -
Immunoprecipitating V5 Tag in Transfected HEK293F cells whole cell lysate
Lane 1: Mouse control IgG2a instead of CSB-MA000161M1m in Transfected HEK293 cells whole cell lysate
Lane 2: CSB-MA000161M1m (2.5μl) + Transfected HEK293F cells whole cell lysate (500μg)
Lane 3: Transfected HEK293F cells whole cell lysate (20μg)
For western blotting, the blot was detected with CSB-MA000161M1m at 1:3000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CUSABIO貨號：CSB-MA000161M1m V5-Tag單克隆抗體是一款針對V5表位標(biāo)簽設(shè)計(jì)的高特異性科研試劑，適用于檢測重組蛋白中的V5標(biāo)簽。V5-Tag作為分子生物學(xué)研究中常用的短肽標(biāo)簽（14個(gè)氨基酸），可特異性標(biāo)記重組蛋白以便于追蹤、純化和功能研究。本產(chǎn)品經(jīng)嚴(yán)格驗(yàn)證具有卓越的物種廣譜性，可在ELISA實(shí)驗(yàn)中實(shí)現(xiàn)精準(zhǔn)定量檢測，通過Western Bloting（WB）清晰呈現(xiàn)目標(biāo)條帶，并支持免疫熒光（IF）進(jìn)行亞細(xì)胞定位分析，同時(shí)適用于免疫共沉淀（IP）實(shí)驗(yàn)以研究蛋白質(zhì)相互作用。抗體采用雜交瘤技術(shù)制備，批次穩(wěn)定性優(yōu)異，可滿足基因表達(dá)調(diào)控、蛋白質(zhì)相互作用網(wǎng)絡(luò)構(gòu)建、轉(zhuǎn)基因生物模型驗(yàn)證等科研需求。該產(chǎn)品兼容哺乳動物、昆蟲等多種表達(dá)系統(tǒng)，是研究重組蛋白表達(dá)效率、亞細(xì)胞定位及功能機(jī)制的理想工具，廣泛應(yīng)用于分子生物學(xué)、細(xì)胞生物學(xué)及蛋白質(zhì)組學(xué)領(lǐng)域的基礎(chǔ)研究。
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基因名：V5-Tag
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宿主：Mouse
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反應(yīng)種屬：All
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免疫原：GKPIPNPLLGLDST synthetic peptide conjugate to KLH
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：IgG2a
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純化方式：>95%, Protein A purified
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克隆號：9E8B7
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IF, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:5000-1:1280000 IF 1:100-1:200 IP 2μl-5μl -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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