HA-Tag Monoclonal Antibody
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中文名稱(chēng)：HA-Tag鼠單克隆抗體
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貨號(hào)：CSB-MA000158
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規(guī)格：￥1090
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圖片：
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IP antibody use: 5µg HA Mouse IgG1 per ml Lysate, WB 1:5000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with HA-tag fusion protein
Lane 3: IP (untransfted 293 + anti-HA mAb + Protein G agarose)
Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 5: IP (transfected 293 + anti-HA mAb + Protein G agarose)
Lane 6: IP (transfected 293 + Protein G)
Lane 7: Recombinant protein (E.coli) -
IF analysis of 293 cells transfected with a HA-tag protein,1:2000 dilution (blue DAPI, red anti-HA)
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0.5µg HA fusion protein + Primary antibody dilution at 1:10000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：本產(chǎn)品為高特異性小鼠源HA-Tag單克隆抗體（CUSABIO貨號(hào)：CSB-MA000158），針對(duì)來(lái)源于流感病毒血凝素（HA）的表位標(biāo)簽設(shè)計(jì)，廣泛應(yīng)用于分子生物學(xué)研究中融合蛋白的追蹤與檢測(cè)。該抗體經(jīng)親和層析純化制備，可識(shí)別重組蛋白中HA標(biāo)簽的YPYDVPDYA線性表位，在Western Blot實(shí)驗(yàn)中能有效檢測(cè)10-150 kDa范圍內(nèi)的標(biāo)記蛋白，免疫熒光實(shí)驗(yàn)適用細(xì)胞樣本的定位觀察，免疫沉淀實(shí)驗(yàn)可特異性富集目標(biāo)蛋白復(fù)合物。經(jīng)嚴(yán)格驗(yàn)證適用于ELISA、WB、IF、IP等實(shí)驗(yàn)體系，其廣泛物種兼容性支持哺乳動(dòng)物、昆蟲(chóng)等多種表達(dá)系統(tǒng)的HA標(biāo)簽蛋白檢測(cè)。科研人員可將其應(yīng)用于基因表達(dá)調(diào)控研究、蛋白互作網(wǎng)絡(luò)解析、重組蛋白表達(dá)驗(yàn)證等基礎(chǔ)研究領(lǐng)域，尤其適合需要高靈敏度檢測(cè)的轉(zhuǎn)染細(xì)胞或原核表達(dá)系統(tǒng)樣本。
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宿主：Mouse
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反應(yīng)種屬：N/A
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免疫原：Synthetic Peptide
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標(biāo)記方式：Non-conjugated
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抗體亞型：IgG
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克隆號(hào)：26E2
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IF, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IF 1:50-1:200 IP 1:200-1:2000 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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