Recombinant Xenopus laevis Transitional endoplasmic reticulum ATPase (vcp), partial

中文名稱：

有爪蟾蜍vcp重組蛋白
貨號：

CSB-YP025813XBE
規格：
來源：

Yeast
其他：

中文名稱：

有爪蟾蜍vcp重組蛋白
貨號：

CSB-EP025813XBE
規格：
來源：

E.coli
其他：

中文名稱：

有爪蟾蜍vcp重組蛋白
貨號：

CSB-EP025813XBE-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

有爪蟾蜍vcp重組蛋白
貨號：

CSB-BP025813XBE
規格：
來源：

Baculovirus
其他：

中文名稱：

有爪蟾蜍vcp重組蛋白
貨號：

CSB-MP025813XBE
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

vcp
Uniprot No.：

P23787
別名：

vcpTransitional endoplasmic reticulum ATPase; TER ATPase; EC 3.6.4.6; 15S Mg(2+)-ATPase p97 subunit; p97; Valosin-containing protein; VCP
種屬：

Xenopus laevis (African clawed frog)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the nuclear envelope, and of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. Involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. Plays a role in the regulation of stress granules (SGs) clearance process upon arsenite-induced response. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites and promotes the recruitment of tp53bp1 at DNA damage sites. Together with sprtn metalloprotease, involved in the repair of covalent DNA-protein cross-links (DPCs) during DNA synthesis. Involved in interstrand cross-link repair in response to replication stress by mediating unloading of the ubiquitinated CMG helicase complex. Enhances cell cycle progression and inhibits apoptosis at low temperatures. Essential for the maturation of ubiquitin-containing autophagosomes and the clearance of ubiquitinated protein by autophagy. Acts as a negative regulator of type I interferon production by promoting ubiquitination of ddx58/rig-i. May play a role in the ubiquitin-dependent sorting of membrane proteins to lysosomes where they undergo degradation. May more particularly play a role in caveolins sorting in cells. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway.
基因功能參考文獻：
1. VCP removes sterically trapped Ku70/80 rings from DNA in double-strand break repair. PMID: 27716483
2. Findings indicate that p97 plays a conserved role in dismantling the CMG helicase complex during different cellular events, but that distinct regulatory signals ultimately control when and where unloading takes place. PMID: 27644328
3. p97 is an essential regulator of DNA damage-dependent CDT1 destruction PMID: 21981919
4. CDC-48/p97 coordinates CDT-1 degradation with GINS chromatin dissociation to ensure faithful DNA replication PMID: 21981920
5. data reveal an essential pathway that regulates reformation of the nucleus after mitosis and defines ubiquitin-dependent protein extraction as a common mechanism of Cdc48/p97 activity also during nucleus formation PMID: 18097415
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亞細胞定位：

Cytoplasm, cytosol. Endoplasmic reticulum. Nucleus. Cytoplasm, Stress granule.
蛋白家族：

AAA ATPase family
組織特異性：

Expressed in at least oocytes, liver and kidney (at protein level).
數據庫鏈接：

KEGG: xla:380491

UniGene: Xl.8775