Recombinant Translocated intimin receptor Tir (tir), partial

中文名稱：

大腸桿菌O157：H7tir重組蛋白
貨號：

CSB-YP752031EOD
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌O157：H7tir重組蛋白
貨號：

CSB-EP752031EOD
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌O157：H7tir重組蛋白
貨號：

CSB-EP752031EOD-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌O157：H7tir重組蛋白
貨號：

CSB-BP752031EOD
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌O157：H7tir重組蛋白
貨號：

CSB-MP752031EOD
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

tir
Uniprot No.：

Q7DB77
別名：

tir; espE; Z5112; ECs4561; Translocated intimin receptor Tir; Secreted effector protein Tir
種屬：

Escherichia coli O157:H7
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Multifunctional protein that is required for efficient pedestal formation in host epithelial cells during infection. The extracellular region acts as a receptor for bacterial intimin, allowing the bacterium to attach tightly to the host-cell surface. Simultaneously, the intracellular region initiates a signaling cascade in the host cell, which leads to actin polymerization and formation of actin pedestals at the sites of bacterial adhesion. In strain EDL933, acts via the effector protein EspF(U), in a phosphotyrosine- and NCK-independent manner. Tir binds to host BAIAP2, which mediates association with EspF(U) and leads to stimulation of actin polymerization.
基因功能參考文獻：
1. identified a 6 amino acid (519-524) carboxy-terminal region which was required for efficient Tir secretion and translocation; data suggest that this region may play a role in maintaining EHEC O157:H7 Tir in a secretion-competent conformation PMID: 15686835
2. localizes at or near the site of enterohemorrhagic Escherichia coli O157:H7 adherence to enterocytes in piglets, neonatal calves, and mice. PMID: 16428775
亞細胞定位：

Secreted. Host cell membrane; Multi-pass membrane protein. Note=Secreted via the type III secretion system (TTSS). Released into the host cytoplasm via TTSS and then independently inserts into the plasma membrane from a cytoplasmic location. In host cells, localizes to the tip of the actin pedestal.
蛋白家族：

Tir receptor family
數據庫鏈接：

KEGG: ece:Z5112

STRING: 155864.Z5112