Recombinant Sheep Bone morphogenetic protein 15 (BMP15)

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中文名稱：

Recombinant Sheep Bone morphogenetic protein 15(BMP15)
品名簡(jiǎn)稱：

Recombinant Sheep BMP15 protein
貨號(hào)：

CSB-EP002735SH
說(shuō)明書：

MSDS Datasheet
規(guī)格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

BMP15
Uniprot No.：

Q9MZE2
別名：

(BMP-15)(Growth/differentiation factor 9B)(GDF-9B)
種屬：

Ovis aries (Sheep)
蛋白長(zhǎng)度：

Full Length of Mature Protein
來(lái)源：

E.coli
分子量：

21.3 kDa
表達(dá)區(qū)域：

269-393aa
氨基酸序列

QAGSIASEVPGPSREHDGPESNQCSLHPFQVSFQQLGWDHWIIAPHLYTPNYCKGVCPRVLHYGLNSPNHAIIQNLVSELVDQNVPQPSCVPYKYVPISILLIEANGSILYKEYEGMIAQSCTCR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

May be involved in follicular development. Oocyte-specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth.
基因功能參考文獻(xiàn)：
1. activin can explain that the effects of BMP15 mutations differ when present in different genetic backgrounds PMID: 28069901
2. BMP15 mutation is associated with prolificacy and female sterility. PMID: 28506298
3. Studied polymorphism of BMP15 gene exon 1 using single strand conformational polymorphism (SSCP) and direct DNA sequencing methods in 170 Mehraban and Lori sheep ewes. PMID: 27565869
4. study concluded that growth differentiation factor 9 and bone morphogenetic protein 15 follow stage-specific pattern of expression during the in vivo development of ovarian follicles in sheep, and in vitro culture altered these changes PMID: 26474685
5. two novel mutations in the BMP15 gene are associated with increased litter size and ovulation rate PMID: 23637641
6. Combined effect analyses indicated an extremely significant interaction between the random combinations of BMPR-IB, BMP-15 and FSHR genes on litter size. PMID: 25993301
7. A higher number of oocytes proven to be competent for in vitro development and embryo survival after transfer are recovered from R+ ewes, despite the lower mean size of their follicles at puncture. PMID: 23438026
8. The Lleyn breed was the most likely source of the BMP15 FecX(G) and GDF9 FecG(H) mutations in Belclare and Cambridge sheep, and the BMP15 FecX(B) mutation came from the High Fertility line developed from prolific ewes in commercial flocks in Ireland. PMID: 23301039
9. Studies indicate that in BMP15-Knockout sheep, oocytes die as follicles start to grow. PMID: 23417416
10. DNA polymorphism studies of BMP 15 gene in Corriedale and local Kashmir valley sheep (Ovis aries). PMID: 22425967
11. A mutation in the bone morphogenetic protein 15 gene (FecXR allele) causes increased prolificity in heterozygous and sterility in homozygous ewes. PMID: 21622876
12. It is concluded that the mutation in the BMP15 gene in I+ ewes leads to an earlier acquisition of LH responsiveness by granulosa cells in a greater proportion of follicles and this accounts for the small higher ovulation-rate in these animals. PMID: 19535491
13. Data suggest an interaction between BMP15 levels and nutritional signals in the follicle to control ovulation rate; studies involved heterozygous Inverdale ewes. PMID: 21871206
14. investigation of regulation of gene expression of BMP15 in ovulation: Higher ovulation rate in BB sheep (homozygous for mutation in BMPR1B) is due, at least in part, to low oocyte-derived BMP15 mRNA levels. PMID: 21474605
15. 11-point mutations of BMP1B, BMP15, and GDF9 genes of 97 Bonpala ewes were genotyped. PMID: 21774623
16. A significant association was found between BMP15 gene HinfI polymorphism and litter size in fat-tailed sheep. The heterozygous genotype showed higher litter size. PMID: 21327517
17. These heterozygous genotypes resulted in higher ovulation rates, illustrating that one copy of each of the BMP15 and GDF9 mutations had equivalent effects on the ovulation rate. PMID: 19144040
18. BMP15 has an essential role in ovarian folliculogenesis and the control of ovulation rate in sheep. PMID: 17038554
19. Small Tailed Han ewes carrying mutations in both BMPR-IB and BMP-15 genes had greater litter size than those with either mutation alone. PMID: 17040942
20. sequence of the complete coding region of BMP15 gene and determination of the patterns of expression of mRNAs encoding BMP15 PMID: 17715428
21. in BMP15, this new mutation is associated with increased prolificacy and sterility in heterozygous and homozygous ewes respectively PMID: 18355397
22. Expression patterns of 4 maternal effect genes (ZAR1, MATER, GDF9, and BMP15) were determined in ovine oocytes and in vitro-produced preimplantation embryos [ZAR1]. PMID: 19007555
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亞細(xì)胞定位：

Secreted.
蛋白家族：

TGF-beta family
數(shù)據(jù)庫(kù)鏈接：

KEGG: oas:100141303

UniGene: Oar.16287