Recombinant Saccharomyces cerevisiae Replication factor C subunit 1 (RFC1), partial

中文名稱：

釀酒酵母RFC1重組蛋白
貨號：

CSB-YP019588SVG
規格：
來源：

Yeast
其他：

中文名稱：

釀酒酵母RFC1重組蛋白
貨號：

CSB-EP019588SVG
規格：
來源：

E.coli
其他：

中文名稱：

釀酒酵母RFC1重組蛋白
貨號：

CSB-EP019588SVG-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

釀酒酵母RFC1重組蛋白
貨號：

CSB-BP019588SVG
規格：
來源：

Baculovirus
其他：

中文名稱：

釀酒酵母RFC1重組蛋白
貨號：

CSB-MP019588SVG
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

RFC1
Uniprot No.：

P38630
別名：

RFC1; CDC44; YOR217W; YOR50-7; Replication factor C subunit 1; Replication factor C1; Activator 1 95 kDa subunit; Cell division control protein 44
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Component of the ATP-dependent clamp loader RFC complex for the POL30/PCNA homotrimer DNA clamp. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Replication factor C (RFC or activator 1) complex acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment.
基因功能參考文獻：
1. DNA polymerase epsilon outcompetes double-stranded DNA for recruiting Ctf18-RFC to the replisome. PMID: 29225079
2. Data show that ATP binding rates and ATP-dependent conformational changes in replication factor C (RFC) are fast relative to proliferating cell nuclear antigen (PCNA) binding rates. PMID: 25450506
亞細胞定位：

Nucleus.
蛋白家族：

Activator 1 large subunit family
數據庫鏈接：

KEGG: sce:YOR217W

STRING: 4932.YOR217W