Recombinant Rat Protein DJ-1 (Park7), partial

中文名稱：

大鼠Park7重組蛋白
貨號(hào)：

CSB-YP017454RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

大鼠Park7重組蛋白
貨號(hào)：

CSB-EP017454RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

大鼠Park7重組蛋白
貨號(hào)：

CSB-EP017454RA-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Park7重組蛋白
貨號(hào)：

CSB-BP017454RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Park7重組蛋白
貨號(hào)：

CSB-MP017454RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Park7
Uniprot No.：

O88767
別名：

Park7; Cap1; Protein/nucleic acid deglycase DJ-1; EC 3.1.2.-; EC 3.5.1.-; EC 3.5.1.124; Contraception-associated protein 1; Protein CAP1; Fertility protein SP22; Maillard deglycase; Parkinson disease protein 7 homolog; Parkinsonism-associated deglycase; Protein DJ-1; DJ-1
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
表達(dá)區(qū)域：

-
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage. Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Also displays an apparent glyoxalase activity that in fact reflects its deglycase activity. Plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease; functions probably related to its primary function. It is involved in neuroprotective mechanisms like the stabilization of NFE2L2 and PINK1 proteins, male fertility as a positive regulator of androgen signaling pathway as well as cell growth and transformation through, for instance, the modulation of NF-kappa-B signaling pathway. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. Required for correct mitochondrial morphology and function as well as for autophagy of dysfunctional mitochondria. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Regulates astrocyte inflammatory responses, may modulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells. In pancreatic islets, involved in the maintenance of mitochondrial reactive oxygen species (ROS) levels and glucose homeostasis in an age- and diet dependent manner. Protects pancreatic beta cells from cell death induced by inflammatory and cytotoxic setting. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Metal-binding protein able to bind copper as well as toxic mercury ions, enhances the cell protection mechanism against induced metal toxicity. In macrophages, interacts with the NADPH oxidase subunit NCF1 to direct NADPH oxidase-dependent ROS production, and protects against sepsis.
基因功能參考文獻(xiàn)：
1. DJ-1 is necessary for Resveratrol-mediated cardioprotective effects. PMID: 29287203
2. DJ1 -/- rats cross a tapered balance beam with significantly decreased speed of traversal PMID: 29109055
3. The work revealed that preserving mitochondrial complex I activity and subsequently inhibiting mitochondrial ROS generation could be a novel mechanism by which DJ-1 mediates the cardioprotection of DHP against H/R-induced oxidative stress damage. PMID: 29431188
4. chaperone-mediated autophagy protects mitochondrial function by degrading nonfunctional PARK7 and maintaining its homeostasis, and dysregulation of this pathway may contribute to the neuronal stress and death in Parkinson disease pathogenesis PMID: 27171370
5. Low DJ-1 expression is associated with pulmonary arterial hypertension. PMID: 29069575
6. Results suggest that overexpression of DJ-1 improves mitochondrial function, at least in part, through a mechanism involving Akt phosphorylation on threonine 308. PMID: 26913805
7. DJ-1 activates autophagy to alleviate cardiac hypertrophy. PMID: 28941803
8. The present study identified DJ-1 as an essential mediator responsible for the beneficial effects of the late phase of ischemic preconditioning in vivo. In addition ischemic preconditioning exerts delayed cardioprotective effects by a hitherto unrecognized underlying mechanism; the attenuation of ischemia/reperfusion -induced oxidative stress via the upregulation of DJ-1. PMID: 28035392
9. Data show that high glucose (HG) for 48 h and then exposed to hypoxia for 4 h and reoxygenation for 2 h significantly decreased cell viability in NRK-52E proximal tubular epithelial cells accompanied by a decrease in DJ-1-PARK7 Protein (DJ1) expression. PMID: 27430285
10. Cardioprotection in a cellular model of hypoxic preconditioning is abolished with knockdown of DJ-1. PMID: 25915512
11. Data indicate that females showed greater susceptibility towards developing streptozotocin-induced diabetes because of lower insulin secretion and higher blood glucose levels. PMID: 25726699
12. DJ-1 plays an important role in caffeic acid-mediated protection against ketoprofen-induced oxidative damage in the GI mucosa. PMID: 25037998
13. phenotypic characterization of Pink1, DJ-1 and Parkin recessive gene knockout Parkinson's disease model; results show inactivation of Pink1 or DJ-1 genes produces progressive neurodegeneration and behavioral deficits, suggesting these recessive genes may be essential for the survival of substantia nigra dopaminergic neurons PMID: 24969022
14. distinctive changes in the expression of dopamine presynaptic markers and receptors in the striatum may reflect different compensatory regulation of dopamine system in DJ-1 versus Pink1 knockout rat models of familial parkinson's disease PMID: 24157858
15. Downregulation of cardiac DJ-1 may be responsible for the compromised diabetic heart responsiveness to Ischemia postconditioning cardioprotection. PMID: 24303086
16. Results show DJ-1 protein associated with lipid rafts. This novel function of DJ-1 in lipid rafts, may contribute the pathogenesis of Parkinson's Disease. PMID: 23847046
17. The results of this study suggested that DJ-1 is closely associated with the differentiation of neural stem cells. PMID: 22613231
18. Electrophilic p-quinone formed from 6-hydroxydopamine induces DJ-1 oxidation by decreasing intracellular glutathione. PMID: 22132160
19. DJ-1 induces Akt/PRAS40/TORC1-mediated hypertrophy in response to high glucose. PMID: 21426932
20. The DJ-1 acts with PINK1 in a common pathway to regulate NMDAr-mediated neuronal death. PMID: 20698836
21. DJ-1 protein is predominantly expressed by neurons but can also be detected in astrocytes. PMID: 16047164
22. SP22 was localized to specific cell types within the anterior pituitary gland. PMID: 16246899
23. DJ-1 mRNA expression was restricted mainly to neurons in all brain regions analyzed. PMID: 17599367
24. expression of SP22 in the rat ovary is most prevalent in the corpora lutea and increased during pregnancy PMID: 17609507
25. DJ-1 protects through alleviation of oxidative stress PMID: 18003894
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亞細(xì)胞定位：

Cell membrane; Lipid-anchor. Cytoplasm. Membrane raft. Nucleus. Mitochondrion. Endoplasmic reticulum.
蛋白家族：

Peptidase C56 family
組織特異性：

Ubiquitous. Detected on epididymal sperm. Highly expressed in testis and prostate. Detected at lower levels in heart, lung, brain, liver, kidney, seminal vesicle, caput and corpus epididymis.
數(shù)據(jù)庫鏈接：

KEGG: rno:117287

STRING: 10116.ENSRNOP00000024711

UniGene: Rn.30105