Recombinant Rat Glucagon receptor (Gcgr), partial (Active)

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品名簡稱：

Recombinant Rat Gcgr protein, partial (Active)
貨號：

CSB-MP009316RA1
說明書：

MSDS Datasheet
規格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Rat Gcgr at 2 μg/ml can bind Anti-Gcgr recombinant antibody (CSB-RA009316MA1HU). The EC₅₀ is 29.63-35.34 ng/mL. Biological Activity Assay
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產品詳情

純度：

Greater?than?90%?as?determined?by?SDS-PAGE.
內毒素：

Less?than?1.0?EU/ug?as?determined?by?LAL?method.
生物活性：

Measured by its binding ability in a functional ELISA. Immobilized Rat Gcgr at 2 μg/mL can bind Anti-Gcgr recombinant antibody (CSB-RA009316MA1HU). The EC50 is 29.63-35.34 ng/mL.
基因名：

Gcgr
Uniprot No.：

P30082
別名：

Glucagon receptor; GL-R; Gcgr
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
來源：

Mammalian cell
分子量：

14.4 kDa
表達區域：

27-137aa
氨基酸序列

AQVMDFLFEKWKLYSDQCHHNLSLLPPPTELVCNRTFDKYSCWPDTPPNTTANISCPWYLPWYHKVQHRLVFKRCGPDGQWVRGPRGQSWRDASQCQMDDDEIEVQKGVAK
蛋白標簽：

C-terminal 10xHis-tagged
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized?from?a?0.2?μm?filtered?PBS,?6%?Trehalose,?pH?7.4
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.
基因功能參考文獻：
1. The vasodilatation due to glucagon evokes via the glucagon-receptor, but also via the receptor for GLP-1, and it is endothelium-independent. PMID: 26975347
2. Significant higher GluR density (mRNA and proteins) following exercise and fasting than in the control group. PMID: 23918690
3. Gln(3) residue of glucagon did not interact with the equivalent binding pocket as the Asp(3) residue of vasoactive intestinal peptide or secretin PMID: 11853547
4. A model is proposed for glucagon receptor activation whereby glucagon first interacts with the receptor N-terminal domain followed by more specific interactions between the N-terminal half of the peptide and the first extracellular loop of the receptor. PMID: 12269822
5. glucagon were unaffected by the GLP-1 receptor antagonist exendin-(9-39) but abolished by des-His1-[Glu9]-glucagon-amide, a specific blocker of the glucagon receptor PMID: 15459251
6. The present results indicate that the feeding of an HF diet is associated with a reduction in plasma membrane hepatic glucagon receptor density and G(s)alpha protein content, which is not attenuated by a 30-min exercise bout. PMID: 15687107
7. within hepatic target cells and consequent to glucagon-mediated internalization of the serine-phosphorylated glucagon receptor and the Gsalpha protein, extended signal transduction may occur in vivo at the locus of the endo-lysosomal apparatus PMID: 17010343
8. High fat diet induced obesity is associated with a desensitization and destruction of the hepatic GR PMID: 17053032
9. These results suggest that glucagon stimulates acute release of both forms of ghrelin and thereafter upregulates synthesis and release of desacyl-ghrelin in the rat stomach. PMID: 17462598
10. The actin cytoskeleton, beta-arrestin1, beta-arrestin2, and the glucagon receptor's carboxyl terminus are involved in its recycling. PMID: 18787074
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亞細胞定位：

Cell membrane; Multi-pass membrane protein.
蛋白家族：

G-protein coupled receptor 2 family
數據庫鏈接：

KEGG: rno:24953

STRING: 10116.ENSRNOP00000051845

UniGene: Rn.11225