Recombinant Rat Cyclin-dependent kinase inhibitor 2A, isoform 1 (Cdkn2a)

中文名稱：

Recombinant Rat Cyclin-dependent kinase inhibitor 2A, isoform 1(Cdkn2a)
貨號：

CSB-YP886427RA
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Rat Cyclin-dependent kinase inhibitor 2A, isoform 1(Cdkn2a)
貨號：

CSB-EP886427RA
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Rat Cyclin-dependent kinase inhibitor 2A, isoform 1(Cdkn2a)
貨號：

CSB-EP886427RA-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Rat Cyclin-dependent kinase inhibitor 2A, isoform 1(Cdkn2a)
貨號：

CSB-BP886427RA
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Rat Cyclin-dependent kinase inhibitor 2A, isoform 1(Cdkn2a)
貨號：

CSB-MP886427RA
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Cdkn2a
Uniprot No.：

Q9R0Z3
別名：

Cdkn2a; P16ink4aCyclin-dependent kinase inhibitor 2A; Cyclin-dependent kinase 4 inhibitor A; CDK4I; p16-INK4a; p16; p16-INK4
種屬：

Rattus norvegicus (Rat)
蛋白長度：

full length protein
表達區域：

1-159
氨基酸序列

MESSADRLAR AAALGREHEV RALLEAGASP NAPNTFGRTP IQVMMMGNVK VAALLLSYGA DSNCEDPTTL SRPVHDAARE GFLDTLVVLH QAGARLDVRD AWGRLPLDLA LERGHHDVVR YLRYLLSSAG NVSRVTDRHN FCSSTPRCLG LRGQPPKQR
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
基因功能參考文獻：
1. Our findings indicated that p16 alteration is a common event in the carcinogenesis of this model and that the mutation pattern is analogous to that of human lesions. PMID: 25865695
2. These findings indicate that p16(INK4a) plays a significant role in the inhibition of DNA synthesis. PMID: 24190239
3. Ezh2 knockdown induced changes in DNA methylation of the p16INK4a gene and premature senescence in mesenchymal stem cells. PMID: 22873822
4. Data show that an accumulation of p16(INK4a)-positive senescent astrocytes may link increased age and increased risk for sporadic Alzheimer's disease (AD). PMID: 22984612
5. hIGF-1 gene therapy improved the expression of rIGF-1 and PCNA protein in myocardium, and also suppressed the expression of p16(INK4a) protein post-infarction. PMID: 18704299
6. Maternal high fat exposure represses p16 (INK4a) gene expression in the mammary gland of offspring through changes of histone modifications and HDAC3 binding activity within the regulatory regions of the p16 (INK4a) gene. PMID: 22395468
7. Subchronic grape juice concentrate administration was able to modulate cell cycle control by downregulation of p16 immunoexpression in high fat diet-induced liver steatosis in rats. PMID: 22320862
8. In obesity prone rats, the increase of p16(INK4a) was associated with the higher acetylation levels of histone H4 at the p16(INK4a) promoter and coding region and lower methylation level of histone H3 lysine-27 in the p16(INK4a) coding region. PMID: 22194422
9. Chromatin immunoprecipitation (ChIP) assay demonstrated that the altered p16 mRNA level and transcription rate in LP offspring resulted from histone code changes, including the reduced acetylation of histone H4 and the dimethylation of histone H3 PMID: 20934317
10. These findings suggest that loss of function or decrease of RAR-beta2, RASSF1A, and CDKN2A, as well as the hypermethylation of 5' region of these genes, are related with nickel exposure. PMID: 21565688
11. Chromosome aberrations of this protein in radiation-induced and spontaneous rat mammary carcinomas. PMID: 20681787
12. iron-induced peritoneal mesothelioma associated with CDKN2A/2B homozygous deletion, supporting the hypothesis that oxidative stress by iron overload is a major cause of CDKN2A/2B homozygous deletion PMID: 20065947
13. p16 (INK4A) locus is specifically vulnerable to oxidative damage, leading to its allelic loss within weeks, presumably due to a deficiency in the replication of both the alleles PMID: 11839561
14. Mts1-expressing astrocytes play a role in degenerative events in the CNS white matter, interact with phagocytic microglia/macrophages, and regulate CNS cell migration and differentiation. PMID: 11870873
15. The inhibitory effect of methylation on gene transcription was confirmed through RT-PCR expression studies in which p16 gene expression was 30-60-fold lower in methylated than unmethylated tumors PMID: 11872642
16. methylation status of the 5' region was more closely correlated with p16 expression than that of the exon 1 alpha and analysis of the methylation status is useful in examining p16 silencing in various rat tumors. PMID: 12417039
17. The p16 protein is mainly orientated in the cell nucleus and sporadically orientated in the cytoplasm. PMID: 15144691
18. Inactivation by hypermethylation of the promoter region of p16INK4A gene was related to carcinogenesis of oral cancer. PMID: 15201967
19. Ink4a and Arf have roles in mammalian aging PMID: 15520862
20. Both of these closely spaced ATG start codons can be utilized as a translational start codon to produce a nucleolar-localized ARF protein which can induce a p53-dependent inhibition of cell division PMID: 16760664
21. In rats, the non-genotoxic carcinogen phenobarbital produced reversible changes in the methylation region of the p16 gene. PMID: 17706854
22. INK4a/ARF gene expression is reduced in stem-like sphere cells from rat sarcomas PMID: 17761140
23. the expression of cell cycle regulator p16 is not affected by diabetes in the studied rat model of oral oncogenesis PMID: 18019407
24. The 2-methoxyestradiol-induced apoptosis of rat osteosarcoma and rat malignant fibrous histiocytoma cells was accompanied by caspase-3 activation through p16 induction. PMID: 18396777
25. Hypertension induces cellular senescence via p16(INK4a), possibly through p38, thereby contributing to hypertensive target organ damage. PMID: 18504326
26. Ink4a/Cdk4/Rb1 pathway deregulation, more than Arf/Mdm2/Tp53 pathway, has a major role in the development of these tumors through p16(Ink4a) deregulation. PMID: 18656278
27. PPARalpha trans-repression involved the recruitment of the retinoblastoma (RB) family proteins p107 and p130 to the TERT promoter resulting in impaired E2F-1 binding, an effect that was dependent on p16. PMID: 18818403
28. Results suggest that p16CDKN2A mutations in exon 2 are not involved in the multistep tongue carcinogenesis nduced by 4NQO. PMID: 19255859
29. betaine affects p16 and c-myc DNA methylation and mRNA expression in a chemical induced rat liver cancer model PMID: 19642983
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亞細胞定位：

Cytoplasm. Nucleus.
蛋白家族：

CDKN2 cyclin-dependent kinase inhibitor family
組織特異性：

Expressed in spleen, liver and lung. Not detected in kidney, colon, stomach or brain.
數據庫鏈接：

KEGG: rno:25163

STRING: 10116.ENSRNOP00000061040

UniGene: Rn.48717