Recombinant Porcine reproductive and respiratory syndrome virus Replicase polyprotein 1ab, partial

中文名稱：

Recombinant Porcine reproductive and respiratory syndrome virus Replicase polyprotein 1ab,partial
貨號：

CSB-YP368831PYZ
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Porcine reproductive and respiratory syndrome virus Replicase polyprotein 1ab,partial
貨號：

CSB-EP368831PYZ
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Porcine reproductive and respiratory syndrome virus Replicase polyprotein 1ab,partial
貨號：

CSB-EP368831PYZ-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Porcine reproductive and respiratory syndrome virus Replicase polyprotein 1ab,partial
貨號：

CSB-BP368831PYZ
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Porcine reproductive and respiratory syndrome virus Replicase polyprotein 1ab,partial
貨號：

CSB-MP368831PYZ
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

N/A
Uniprot No.：

A0MD28
別名：

Replicase polyprotein 1ab; ORF1ab polyprotein) [Cleaved into: Nsp1; EC 3.4.22.-); Nsp1-alpha papain-like cysteine proteinase; EC 3.4.22.-; PCP1-alpha); Nsp1-beta papain-like cysteine proteinase; EC 3.4.22.-; PCP1-beta); Nsp2 cysteine proteinase; EC 3.4.19.12; EC 3.4.22.-; CP2; CP); Non-structural protein 3; Nsp3); Serine protease nsp4; 3CLSP; EC 3.4.21.-; 3C-like serine proteinase; Nsp4); Non-structural protein 5-6-7; Nsp5-6-7); Non-structural protein 5; Nsp5); Non-structural protein 6; Nsp6); Non-structural protein 7-alpha; Nsp7-alpha); Non-structural protein 7-beta; Nsp7-beta); Non-structural protein 8; Nsp8); RNA-directed RNA polymerase; Pol; RdRp; EC 2.7.7.48; Nsp9); Helicase nsp10; Hel; EC 3.6.4.12; EC 3.6.4.13; Nsp10); Non-structural protein 11; Nsp11); Non-structural protein 12; Nsp12)]
種屬：

Porcine reproductive and respiratory syndrome virus (isolate Pig/United States/SD 01-08/2001) (PRRSV)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.; Inhibits host IFN-beta production. Plays a role in the degradation of the host transcriptional activator CREBBP protein. The degradation of host CREBBP which is a key component of the IFN enhanceosome is likely responsible for the inhibition of interferon mediated by Nsp1-alpha. Participates also in the inhibition of host NF-kappa-B activation by counteracting LUBAC-dependent induction of NF-kappa-B. Reduces host NEMO ubiquitination by blocking the interaction between the two LUBAC complex components RNF31 and SHARPIN.; Plays a role in blocking host mRNA nuclear export to the cytoplasm and subversion of host protein synthesis. Additionally, inhibits the interferon-activated JAK/STAT signal transduction by mediating the ubiquitination and subsequent proteasomal degradation of host KPNA1.; Multifunctional protein that acts as a viral protease and as a viral antagonist of host immune response. Cleaves the nsp2/nsp3 site in the viral polyprotein. Displays deubiquitinating activity that cleaves both ubiquitinated and ISGylated products and therefore inhibits ubiquitin and ISG15-dependent host innate immunity. Deubiquitinates also host NFKBIA, thereby interfering with NFKBIA degradation and impairing subsequent NF-kappa-B activation.; Plays a role in the inhibition of the immune response by interacting with host IFITM1. This interaction leads to the proteasomal degradation of the IFN-induced antiviral protein IFITM1.; Cleaves the majority of cleavage sites present in the C-terminus of the polyprotein. Triggers host apoptosis through caspase-3, -8, and -9 activations. Subverts host innate immune responses through its protease activity. Targets the NF-kappa-B essential modulator NEMO and mediates its cleavage. Blocks host interferon beta induction and downstream signaling by cleaving mitochondrial MAVS, dislodging it from the mitochondria. Impairs host defense by cleaving host mRNA-decapping enzyme DCP1A to attenuate its antiviral activity.; Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic.; Plays a role in the inhibition of host STAT3 signaling pathway by inducing the degradation of STAT3.; Responsible for replication and transcription of the viral RNA genome.; Displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.; Plays a role in viral transcription/replication and prevents the simultaneous activation of host cell dsRNA sensors, such as MDA5/IFIH1, OAS, and PKR. Acts by degrading the 5'-polyuridines generated during replication of the poly(A) region of viral genomic and subgenomic RNAs. Catalyzes a two-step reaction in which a 2'3'-cyclic phosphate (2'3'-cP) is first generated by 2'-O transesterification, which is then hydrolyzed to a 3'-phosphate (3'-P). If not degraded, poly(U) RNA would hybridize with poly(A) RNA tails and activate host dsRNA sensors.
亞細胞定位：

[Nsp1]: Host nucleus. Host cytoplasm.; [Nsp1-alpha papain-like cysteine proteinase]: Host nucleus. Host cytoplasm.; [Nsp1-beta papain-like cysteine proteinase]: Host nucleus.; [Nsp2 cysteine proteinase]: Host cytoplasm. Host membrane; Multi-pass membrane protein.; [Non-structural protein 3]: Host membrane; Multi-pass membrane protein.; [Non-structural protein 5-6-7]: Host endoplasmic reticulum. Host membrane; Multi-pass membrane protein.; [Serine protease nsp4]: Host cytoplasm.; [RNA-directed RNA polymerase]: Host cytoplasm. Host cytoplasm, host perinuclear region.; [Helicase nsp10]: Host cytoplasm. Host cytoplasm, host perinuclear region.; [Uridylate-specific endoribonuclease nsp11]: Host cytoplasm. Host nucleus.; [Non-structural protein 12]: Host cytoplasm.
蛋白家族：

Arteriviridae polyprotein family