Recombinant Pig Arachidonate 12-lipoxygenase, 12S-type (ALOX12), partial

中文名稱：

Recombinant Pig Arachidonate 12-lipoxygenase, 12S-type(ALOX12) ,partial
貨號：

CSB-YP001617PI
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Pig Arachidonate 12-lipoxygenase, 12S-type(ALOX12) ,partial
貨號：

CSB-EP001617PI
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Pig Arachidonate 12-lipoxygenase, 12S-type(ALOX12) ,partial
貨號：

CSB-EP001617PI-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Pig Arachidonate 12-lipoxygenase, 12S-type(ALOX12) ,partial
貨號：

CSB-BP001617PI
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Pig Arachidonate 12-lipoxygenase, 12S-type(ALOX12) ,partial
貨號：

CSB-MP001617PI
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

ALOX15
Uniprot No.：

P16469
別名：

ALOX15; Arachidonate 15-lipoxygenase; 15-LOX; EC 1.13.11.33; 12/15-lipoxygenase; Arachidonate 12-lipoxygenase; leukocyte-type; 12-LOX; EC 1.13.11.31; Arachidonate omega-6 lipoxygenase; Erythroid cell-specific 15-lipoxygenase
種屬：

Sus scrofa (Pig)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. It inserts peroxyl groups at C12 or C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing both 12-hydroperoxyeicosatetraenoate/12-HPETE and 15-hydroperoxyeicosatetraenoate/15-HPETE. It may then act on 12-HPETE to produce hepoxilins, which may show proinflammatory properties. Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate. May participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs)like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively downregulate the immune response and have anti-aggregation properties with platelets. Can convert epoxy fatty acids to hydroperoxy-epoxides derivatives followed by an intramolecular nucleophilic substitution leading to the formation of monocyclic endoperoxides. Plays an important role during the maintenance of self-tolerance by peroxidizing membrane-bound phosphatidylethanolamine which can then signal the sorting process for clearance of apoptotic cells during inflammation and prevent an autoimmune response. In addition to its role in the immune and inflammatory responses, this enzyme may play a role in epithelial wound healing in the cornea through production of lipoxin A4 (LXA(4)) and docosahexaenoic acid-derived neuroprotectin D1 (NPD1; 10R,17S-HDHA), both lipid autacoids exhibit anti-inflammatory and neuroprotective properties. Furthermore, it may regulate actin polymerization which is crucial for several biological processes such as the phagocytosis of apoptotic cells. It is also implicated in the generation of endogenous ligands for peroxisome proliferator activated receptor (PPAR-gamma), hence modulating macrophage development and function. It may also exert a negative effect on skeletal development by regulating bone mass through this pathway. As well as participates in ER stress and downstream inflammation in adipocytes, pancreatic islets, and liver. Finally, it is also involved in the cellular response to IL13/interleukin-13.
基因功能參考文獻：
1. The crystal structure of a mammalian 12-lipoxygenase reveals a plausible substrate access channel for oxygen. PMID: 22795085
2. 12/15-Lipoxygenase activity increases the degradation of macrophage ATP-binding cassette transporter G1. PMID: 18635820
3. EPR spectroscopy and electrospray mass spectroscopy reveal distinctive features of the iron site in leukocyte arachidonate 12-lipoxygenase. PMID: 19683507
亞細胞定位：

Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Lipid droplet.
蛋白家族：

Lipoxygenase family
數據庫鏈接：

KEGG: ssc:396971

STRING: 9823.ENSSSCP00000018988

UniGene: Ssc.10974