Recombinant Mouse Vesicle-associated membrane protein 2 (Vamp2), partial

中文名稱：

小鼠Vamp2重組蛋白
貨號：

CSB-YP025781MO1
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

小鼠Vamp2重組蛋白
貨號：

CSB-EP025781MO1
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

小鼠Vamp2重組蛋白
貨號：

CSB-EP025781MO1-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Vamp2重組蛋白
貨號：

CSB-BP025781MO1
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Vamp2重組蛋白
貨號：

CSB-MP025781MO1
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
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靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Vamp2
Uniprot No.：

P63044
別名：

Vamp2; Syb2; Vesicle-associated membrane protein 2; VAMP-2; Synaptobrevin-2
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 668
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Involved in the targeting and/or fusion of transport vesicles to their target membrane. Major SNARE protein of synaptic vesicles which mediates fusion of synaptic vesicles to release neurotransmitters. Essential for fast vesicular exocytosis and activity-dependent neurotransmitter release as well as fast endocytosis that mediates rapid reuse of synaptic vesicles. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1.
基因功能參考文獻(xiàn)：
1. These observations provide evidence that the synaptobrevin-2 transmembrane domain catalyzes the membrane fusion process by its structural flexibility, actively setting the pace of fusion pore expansion. PMID: 27343350
2. Thus, lipid-anchored syb2 provides little or no support for exocytosis, and anchoring syb2 to a membrane by a TMD greatly improves its function PMID: 26663078
3. Vamp2 mutations impair the ability of Munc18-1 to promote trans-SNARE zippering. These mutations inhibit spontaneous as well as evoked neurotransmitter release, providing evidence for the Vamp2-regulating function of Munc18-1 in synaptic exocytosis. PMID: 26572858
4. These results provide a novel molecular mechanism for autocrine negative feedback regulation of insulin secretion. PMID: 26585489
5. Results suggest that side chains in the syb2 transmembrane domain influence the kinetics of exocytosis by perturbing the packing of the surrounding lipids PMID: 26153704
6. Here we report on transgenic mice expressing a ubiquitinated synaptic vesicle protein (Ub(G76V)-GFP-Syb2) that develop progressive degeneration of motor nerve terminals. PMID: 26290230
7. VAMP2 is the major v-SNARE involved in GLUT4 trafficking to the surface of 3T3-L1 adipocytes. PMID: 25501368
8. These results indicate a role for the syb2 TMD in nascent fusion pores, but in a very different structural arrangement from that of the syntaxin transmembrane domains. PMID: 25855187
9. Clustering was dependent on specific interactions of native alpha-synuclein with both synaptobrevin-2/VAMP2 and anionic lipids PMID: 23638301
10. Findings indicate an essential role for VAMP2 in GLP-1 exocytosis from the GLUTag L cell in response to a variety of established secretagogues. PMID: 24356748
11. synaptobrevin2 is expressed in cytotoxic T lymphocytes and exclusively localized on granzyme B-containing lytic granules. PMID: 23385584
12. Data suggest that tryptophans W89/W90 in juxtamembrane region/transmembrane domain of Syb2 act as fusion clamp in chromaffin cells; in mutant protein, alanines A89/A90 promote spontaneous membrane fusion. PMID: 23178715
13. The tryptophan moiety in SybII keeps secretory vesicles in the release-ready state and support a model wherein tryptophan-mediated protein-lipid interactions assist in bridging the apposing membranes before fusion. PMID: 23136435
14. microelectrode array measurements in specific hippocampal subregions of VAMP2(+/-) mice showed significant reductions in potassium-evoked glutamate release PMID: 22183055
15. VAMP2 and VAMP3 are expressed in JG cells, but only VAMP2 is targeted to renin-containing granules and mediates the stimulatory effect of cAMP on renin exocytosis. PMID: 21708949
16. Two copies of spH per synaptic vesicles (SV) fully rescued evoked fusion whereas SVs expressing only one spH were unable to rapidly fuse upon stimulation. PMID: 21844343
17. T3 may promote glucose uptake via enhancing insulin-induced phosphorylation of Akt and subsequent translocations of VAMP2 and glucose transporter GLUT4 in adipocytes. PMID: 21792921
18. multiple SybII actions determine the exquisite temporal regulation of neuronal signaling. PMID: 20685972
19. Results suggest a model in which the positively charged VAMP2 and syntaxin juxtamembrane regions facilitate fusion by bridging the negatively charged vesicle and plasma membrane leaflets. PMID: 19812247
20. essential for two fast synapse-specific membrane trafficking reactions: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid reuse of synaptic vesicles PMID: 15475946
21. may function in catalyzing fusion reactions and stabilizing fusion intermediates but is not absolutely required for synaptic fusion PMID: 11691998
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亞細(xì)胞定位：

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Cell membrane.
蛋白家族：

Synaptobrevin family
組織特異性：

Expressed in the outer plexiform layer of the retina (at protein level).
數(shù)據(jù)庫鏈接：

KEGG: mmu:22318

STRING: 10090.ENSMUSP00000021273

UniGene: Mm.28643