Recombinant Mouse Transcription factor PU.1 (Spi1)

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中文名稱：

小鼠Spi1重組蛋白
品名簡稱：

Recombinant Mouse Spi1 protein
貨號：

CSB-EP022567MO
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

Spi1
Uniprot No.：

P17433
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length
來源：

E.coli
分子量：

38.8 kDa
表達區域：

1-272aa
氨基酸序列

MLQACKMEGFSLTAPPSDDLVTYDSELYQRPMHDYYSFVGSDGESHSDHYWDFSAHHVHNNEFENFPENHFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHTGLSHQVSYMPRMCFPYQTLSPAHQQSSDEEEGERQSPPLEVSDGEADGLEPGPGLLHGETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRLPPH
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
基因功能參考文獻：
1. PU.1 mediates allergic responses in mast cells. PU.1 directly binds to Syk promoter. PMID: 29386516
2. Mice lacking both PU.1 and SpiB in mature B cells do not generate germinal centers and high-affinity antibody after protein immunization. PU.1 and SpiB double-deficient B cells have a survival defect after engagement of CD40 or Toll-like receptors (TLR), despite paradoxically enhanced plasma cell differentiation. PMID: 29127283
3. PU.1 can bind directly to the most-proximal Ets motif, which is essential for the transcriptional function of the mouse OX40L promoter in dendritic cells. PMID: 27708417
4. PU.1 suppresses Sirt1 translation via transcriptional promotion of miR-34a/-29c. PMID: 29162670
5. The analysis points to a critical role for Hoxa9 and PU.1 in distal regulation of c-myb expression in murine myeloid cells during iL-6-induced cell differentiation. PMID: 27607579
6. Data suggest that site-specifically bound PU.1 dimers occupy an extended DNA interface downstream from 5prime-GGAA-3prime core consensus relative to its 1:1 counterpart, thus explaining apparent site size requirement for sequential dimerization of PU.1. PMID: 28790174
7. PU.1 levels affected the expression of mouse orthologs of many Alzheimer's diseas risk genes and the phagocytic activity of mouse microglial cells PMID: 28628103
8. These studies reveal an important role for PU.1 in the regulation of Igkappa transcription and rearrangement and a requirement for PU.1 and Spi-B in B cell development. PMID: 28062693
9. expression of an essential mediator of neutrophil terminal differentiation, the ets transcription factor PU.1, was significantly decreased in Hbb(th3/+) neutrophils in a model of beta-thalassemia PMID: 28325862
10. Moreover, the expression of a cell proliferation marker Ki67 was significantly decreased in tumors from the mice not taking doxycycline, compared with that of tumors from the mice continuously taking doxycycline. The present data strongly suggest that PU.1 functions as a tumor suppressor of myeloma cells in vivo. PMID: 28347818
11. the affinities of two sequence-divergent ETS homologs, PU.1 and Ets-1, to DNA sites harboring a hemi- and fully methylated CpG dinucleotide, were measured. PMID: 27270080
12. this study shows that the generation of central nervous system macrophages relies on the transcription factor PU.1 PMID: 27135602
13. this study shows that PU.1 functions as a positive regulator of CD11c gene expression by directly binding to the Itgax promoter and through transactivation of the Irf4 gene PMID: 28338898
14. Here we demonstrate that the transcription factors SPI1 (PU.1) and HOXC13 synergistically regulate Zfp521 expression, and identify the regions of the Zfp521 promoter required for this transcriptional activity. We also show that SPI1 and HOXC13 activate Zfp521 in a dose-dependent manner. PMID: 27506447
15. Authors report that PU.1 serves as a critical regulator of alternatively activated macrophage(AAM) polarization and promotes the pathological progress of asthmatic airway inflammation. PMID: 26101328
16. findings suggest that Gata1 & PU.1 transcription factors are only executing and reinforcing lineage choice once made. These results challenge the current prevailing model of early myeloid lineage choice PMID: 27411635
17. involved in osteoclast development by transactivating NFATc1 expression via direct binding to the NFATc1 promoter PMID: 26117255
18. GATA1 and PU.1 bind in vitro and in vivo the proximal promoter region of the RPS19 gene which is frequently mutated in Diamond-Blackfan Anemia. PMID: 26447946
19. PU.1 Suppresses Th2 Cytokine Expression via Silencing of GATA3 Transcription in Dendritic Cells PMID: 26361334
20. Taken together, our results suggest that the expression of NDRG2 potentially inhibits osteoclast differentiation and plays a role in modulating the signal transduction pathway responsible for osteoclastogenesis. PMID: 26546825
21. these data indicate an inhibitory role for PU.1 in the function of Tfh cells, germinal centers, and Tfh-dependent humoral immunity. PMID: 26363052
22. Our results suggest that complementary biological functions of PU.1 and Spi-B may be explained by their interaction with a similar set of regions in the genome of B cells. PMID: 25765478
23. PU.1 keeps macrophage-specific genes accessible during differentiation by preventing Polycomb repressive complex 2 binding to transcriptional regulatory elements. PMID: 26012552
24. Nfkb1 transcriptional activation by PU.1 and Spi-B promotes toll-like receptor-mediated B cell proliferation. PMID: 25733685
25. in addition to supporting early T-cell proliferation, PU.1 regulates the timing of activation of the core T-lineage developmental program. PMID: 25846797
26. These findings indicate that PU.1-mediated upregulation of CSF-1R is a critical effector of MLL leukemogenesis. PMID: 25529853
27. The Nogo-B-PirB axis controls macrophage-mediated vascular remodeling. PMID: 24278366
28. these results suggest that PU.1 and Spi-B activate Btk to oppose IL-7 responsiveness in developing B cells. PMID: 25505273
29. Runx-dependent PU.1 chromatin interaction and transcription of PU.1 are essential for both normal and leukemia stem cells. PMID: 25185713
30. miR-155 inhibits PU.1 expression, leading to Pax5 down-regulation and the initiation of the plasma cell differentiation pathway. PMID: 25288398
31. The transcription factors IRF8 and PU.1 negatively regulate plasma cell differentiation. PMID: 25288399
32. Deletion of p53 mice with a homozygous deletion of the upstream regulatory element in PU.1 results in more aggressive acute myeloid leukemia. PMID: 24121269
33. PU.1 overexpression resulted in upregulation of miR-191 and adipogenic inhibition. Likewise, PU.1 overexpression rescued the miR-191 decrease and resisted the adipogenic promotion caused by miR-191 oligonucleotide inhibitor. PMID: 25094047
34. spontaneous leukemias that develop in mice of the mixed 129S2/SvPas and C57BL/6 background of knockout mice arise by a pathway that does not involve biallelic PU.1 mutation. PMID: 25076114
35. our findings demonstrate that PU.1 contributes to the development of MLL leukemia, partially via crosstalk with the MEIS/HOX pathway. PMID: 24445817
36. Identification of a minimal set of DNA sequence and shape features that accurately predict both Pu.1 binding and nucleosome occupancy genome-wide. PMID: 24813947
37. Mice with PU.1 deficiency in T cells were protected from colitis, whereas treatment with antibody to IL-9 suppressed colitis PMID: 24908389
38. the expression control of Tal2 in hematopoietic cells PMID: 24086757
39. Data indicate that cultured Sfpi1(BN/BN) cells expressed elevated messenger RNA transcript and protein levels of E2F1, an important regulator of cell cycle entry. PMID: 24316397
40. Spi-1 inhibits mitochondrial apoptosis in vitro and in vivo through the transcriptional repression of Bim, a proapoptotic factor. PMID: 23852375
41. It was concluded that the conformational change in Runx1 induced by Pin1 represses PU.1 transcription in pre-monocytes and influences the commitment to the monocyte lineage. PMID: 24037986
42. PU.1 Expression Induces -50 kb Irf8 Enhancer Activity and Chromatin Remodeling. PMID: 23623495
43. PU.1 expression repressed genes with nearby adipocyte-specific PPARgamma binding sites, while a common macrophage-adipocyte gene expression program was retained. PMID: 23775123
44. these study analyzed PU.1 and cell cycle regulation in individual cells during early macrophage and B cell development. PMID: 23868921
45. Biosensor-surface plasmon resonance (SPR) reveals a striking kinetic profile for DNA binding by the PU.1 transcription factor domain. At low salt concentrations, it binds high-affinity cognate DNA with a very slow association rate constant. PMID: 23416556
46. Data indicate that FOS, SPI1, KLF10, TFEC, and PRDM16 show robust transcriptional cross-regulation and are often associated with osteoclastogenesis. PMID: 23340137
47. Data conclude that both GM-CSF and FLT3L act through PU.1 to activate the 5.3 Kb CD11c proximal promoter in DCs and to induce differentiation of monocyte-derived DCs. PMID: 23284905
48. A critical role for PU.1 initiates microRNA-142 expression and modulates interleukin (IL)-6 expression in response to Toll-like receptor (TLR)4 signaling. PMID: 23509362
49. Spi-1, Fli-1 and Fli-3 (miR-17-92) oncogenes contribute to a single oncogenic network controlling cell proliferation in friend erythroleukemia. PMID: 23056458
50. Maintenance of Gata3 protein levels by Myb and Notch signaling is linked to the ability to retain T-cell identity in response to PU.1. PMID: 23444353
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相關疾病：

Involved in murine acute Friend erythroleukemia. It is a target region for SFFV proviral insertion.
亞細胞定位：

Nucleus.
蛋白家族：

ETS family
數據庫鏈接：

KEGG: mmu:20375

STRING: 10090.ENSMUSP00000002180

UniGene: Mm.1302