Recombinant Mouse Peptidyl-glycine alpha-amidating monooxygenase (Pam), partial

中文名稱：

小鼠Pam重組蛋白
貨號：

CSB-YP017417MO
規(guī)格：
來源：

Yeast
其他：

中文名稱：

小鼠Pam重組蛋白
貨號：

CSB-EP017417MO
規(guī)格：
來源：

E.coli
其他：

中文名稱：

小鼠Pam重組蛋白
貨號：

CSB-EP017417MO-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Pam重組蛋白
貨號：

CSB-BP017417MO
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Pam重組蛋白
貨號：

CSB-MP017417MO
規(guī)格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Pam
Uniprot No.：

P97467
別名：

PamPeptidyl-glycine alpha-amidating monooxygenase; PAM) [Includes: Peptidylglycine alpha-hydroxylating monooxygenase; PHM; EC 1.14.17.3); Peptidyl-alpha-hydroxyglycine alpha-amidating lyase; EC 4.3.2.5; Peptidylamidoglycolate lyase; PAL)]
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides. Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monoxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate. The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate. Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate (Probable).
基因功能參考文獻：
1. Oxygen Sensitivity of the Peptidylglycine alpha-Amidating Monooxygenase (PAM) in Neuroendocrine Cells PMID: 26296884
2. Disruption of AP-1-dependent late endosomal trafficking diminishes the ability of PAM to retain copper and produce amidated peptides. PMID: 26170456
3. deficits in PAM reversibly affect copper homeostasis, with behavioral and physiological consequences PMID: 24593825
4. Reduced amygdalar expression of Atox-1 and Atp7a in PAM heterozygous mice may lead to reduced synaptic Cu levels, contributing to the behavioral and neurochemical alterations seen in these mice. PMID: 24032518
5. While glycine-extended reaction intermediates accumulated in the PAM(+/-) mice and reflected dietary copper availability, amidated products were far more prevalent under the conditions examined. PMID: 22194882
6. Although the copper-dependent mechanisms through which the endocytic trafficking of PAM alters gene expression have not been elucidated in this review, the cytosolic fragment of PAM identified in pituitary and atrial nuclei is likely to play a key role. PMID: 20648645
7. Cleavage of PAM releases a soluble CD fragment that localizes to the nucleus. Localization of PAM-CD to the nucleus was decreased when PAM-CD with phosphomimetic mutations was examined and when active Uhmk1 was simultaneously overexpressed. PMID: 20573687
8. Altered copper homeostasis in PAM(+/-) mice suggested a role for PAM in the cell type specific regulation of copper metabolism. PMID: 19815072
9. In 12-day-old (P12) mouse pups derived from dams that began Cu deficiency on day 7 of gestation, there was a parallel reduction in brain PAM activity and protein of 40-50%. PMID: 15629125
10. expression analysis of PHM in rats and in mouse cells PMID: 16325307
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亞細胞定位：

Cytoplasmic vesicle, secretory vesicle membrane; Single-pass membrane protein.
蛋白家族：

Peptidyl-alpha-hydroxyglycine alpha-amidating lyase family; Copper type II ascorbate-dependent monooxygenase family
數(shù)據(jù)庫鏈接：

KEGG: mmu:18484

STRING: 10090.ENSMUSP00000095228

UniGene: Mm.441453