Recombinant Mouse Neuronal acetylcholine receptor subunit alpha-7 (Chrna7), partial

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中文名稱：

Recombinant Mouse Neuronal acetylcholine receptor subunit alpha-7 (Chrna7), partial
品名簡稱：

Recombinant Mouse Chrna7 protein, partial
貨號：

CSB-EP005393MOc7
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Chrna7
Uniprot No.：

P49582
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
來源：

E.coli
分子量：

31.4 kDa
表達區域：

23-230aa
氨基酸序列

GEFQRRLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQVLTTNIWLQMSWTDHYLQWNMSEYPGVKNVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNASGHCQYLPPGIFKSSCYIDVRWFPFDVQQCKLKFGSWSYGGWSLDLQMQEADISSYIPNGEWDLMGIPGKRNEKFYECCKEPYPDVTYTVTMRRRT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
基因功能參考文獻：
1. Thus, vagal nerve stimulation of macrophages via alpha7nAchR regulates bone mass by modulating osteoclast formation. PMID: 28349965
2. In this study we used genetic deletion of the alpha7nAChR to confirm our previous inhibitor studies that demonstrated NNK stimulates pancreatitis by activating this receptor. Lastly, we demonstrate that NNK can also stimulate zymogen activation in human acinar cells and thus may play a role in human disease PMID: 29870540
3. Results show that the p38 MAPK signaling pathway could regulate mitochondria Abeta internalization by manipulating the expression of alpha7nAChR. Pretreatment of alpha7nAChR agonist could attenuate these biochemical changes which are tightly associated with Abeta1-42 induced apoptosis. Suggesting there is an endogenous, previously unrecognized cholinergic mechanism to control mitochondria functions and their apoptotic ... PMID: 29128415
4. These findings provide evidence that alpha7nAChR activation mitigates chronic restraint stress -induced neuroinflammation and cell death PMID: 29197398
5. Study demonstrated that activating alpha7nAChR contributed to the inhibition of NLRP3 inflammasome activation through the beta-arrestin-1-mediated manner in experimental auto-immune encephalomyelitis. PMID: 28941191
6. Nicotine stimulates fibroblast proliferation and their expression of collagen type I through alpha7 nAChRs, thereby altering the relative composition of the lung matrix. PMID: 28576119
7. Stimulation of nAChRalpha7 with GTS-21 reduced atherosclerosis, which was associated with dampened splenic myelopoiesis in ApoE knockout mice. PMID: 28858686
8. increased BDNF-TrkB signaling and synaptogenesis in the nucleus accumbens by deletion of alpha7 nAChR plays a key role in depression PMID: 27821848
9. The in vitro findings suggest that GTS-21-induced IL-6 release from muscle is mediated via alpha7AChRs upstream of Stat-3 and -5 pathways and is associated with myonuclear accretion, possibly via MyoD and Pax7 expression. PMID: 28282360
10. alpha7nAChR stimulation reduced neuroinflammation via activation of the JAK2-STAT3 pathway, thereby ameliorating the short- and long-term sequelae after intracerebral hemorrhage . PMID: 28529954
11. In alpha7 nAChR knock-out mice, withdrawal from nicotine does not result in withdrawal-induced inattention. PMID: 28243714
12. MeCP2 is critical for normal function of cholinergic neurons and dysfunction of cholinergic neurons can contribute to numerous neuropsychiatric phenotypes. PMID: 27103432
13. the data suggest involvement of nAChRs of alpha7 subtype in regulation of specific neutrophil functions PMID: 26965141
14. The results suggest that alpha7nAChR has a central role in modulating the response to chronic cigarette smoking that could include altering susceptibility to associated lung diseases including fibrosis and cancer. PMID: 29117258
15. Regulation of expression level and splicing of RIC-3 in brain and in immune cells following inflammation enables regulation of nicotinic acetylcholine receptor functional expression. PMID: 27129882
16. These results support our previous study showing that these PAMs are selective for the alpha7 AChR, and clarify that the procognitive/promnesic/antidepressant activity of PAM-2 is not mediated by other targets. PMID: 27129924
17. The collective results suggest that PNU-282987, an agonist of alpha7nAChR, reduces lipopolysaccharide-induced experimental acute lung injury. PMID: 27729414
18. these results demonstrate that vagus nerve stimulation-mediated attenuation of acute kidney injury and systemic inflammation depends on alpha7nAChR-positive splenocytes. PMID: 27088805
19. Data (including data from studies conducted using knockout mice and SH-SY5Y cell line) suggest that Wnt/beta-catenin signaling is critical effector of Chrna7-associated neuroprotection of dopaminergic neurons in substantia nigra; Parkinson's disease appears to develop without this neuroprotection. PMID: 28551099
20. Cigarette smoke extract and nicotine-induced fibroblast proliferation and collagen content were mediated through alpha7 nicotinic acetylcholine receptors. PMID: 28258105
21. Hippocampal astrocytes sense the wakefulness-dependent activity of septal cholinergic fibers through alpha7nAChR, whose activation drives D-serine release, tuning the gating of NMDA receptors. PMID: 28479102
22. Activation of alpha7nAChR alleviates Ang II-induced vascular smooth muscle cell senescence by promoting NAD(+)-SIRT1 pathway. PMID: 27339462
23. Activation of alpha7 nAChRs increased both prelimbic cortex excitatory and inhibitory signaling, but with differing sensitivities to acetylcholine. Enhancing available acetylcholine results in overall excitation, whereas global activation of alpha7 nAChRs with an exogenous agonist results in overall inhibition PMID: 26921769
24. Study compared the consequences of knocking out the alpha7nAChR on synaptic plasticity in C57/Bl6 and C3H mice, which differ in their basal alpha7nAChR expression levels; homozygous alpha7 deletion in C3H mice, which normally express higher alpha7nAChR levels, resulted in impaired long-term potentiation (LTP) at hippocampal CA1 synapses, while C3H alpha7 heterozygous mice maintained robust LTP. PMID: 27233215
25. Activation of alpha7 nicotinic acetylcholine receptor on mast cells is a mechanism by which nicotine enhances atherosclerosis in ApoE knockout mice. PMID: 27834689
26. We demonstrated that varenicline upregulates expression of LOX-1 and CD36 significantly through alpha7 nAChR, thereby promoting oxLDL uptake in macrophages. PMID: 28202387
27. These data indicated that alpha7-nAChR caused the inhibition of ASPinduced activation of p38 kinase and NFkappa B to inhibit the production of MCP1 and keratinocytederived chemokine. PMID: 27572255
28. These results strongly suggest that activating alpha7nAChR can promote diabetic wound healing by suppressing advanced glycation end products-induced TNF-alpha production, which may be closely associated with the blockage of NF-kappaB activation in macrophages. PMID: 26650489
29. It is concluded that inflammation decreases alpha7 nAChR expression in both mitochondria and cell plasma membrane and makes mitochondria more susceptible to apoptosis induction. PMID: 25887272
30. Chrna7-deficient mice exhibit sustained attention impairments that are reversed by Chrnb2 activation PMID: 26222090
31. The results of this study suggest that activators of nAChRa7 may have therapeutic utility for the treatment of Traumatic brain injury. PMID: 26937017
32. Neuroprotective effect of cellular prion protein (PrPC) is related with activation of alpha7 nicotinic acetylcholine receptor (alpha7nAchR)-mediated autophagy flux. PMID: 26295309
33. results show that preincubation of 3T3-L1 cells with alpha7nAChR agonist GTS-21 significantly reduces ASP-mediated chemokine MCP-1 secretion PMID: 25985797
34. cAMP-dependent protein kinase inhibits alpha7 nicotinic receptor activity in layer 1 cortical interneurons through activation of D1/D5 dopamine receptors PMID: 25990637
35. alpha7nAChR has a role in the lipopolysaccharide-stimulated sinomenine anti-inflammatory effect on macrophages PMID: 25895149
36. we have described the impact of a point mutation in the nicotinic acetylcholine receptor alpha7 on the inflammatory response by the lung to intranasal LPS PMID: 25803612
37. Findings provide evidence to link activation of alpha7 nAChRs to a cAMP rise via AC1, which defines a new signaling pathway employed by alpha7 nAChRs PMID: 25937212
38. Cell surface localization of alpha7-nAChR in the brain of 5xFAD mouse, an animal model of Alzheimer's disease, increased in an age -dependent manner in association with increased accumulation of ABETA 1-42 in the plasma membrane component. PMID: 26522221
39. Chrna7 KO mice had significantly decreased blood-brain barrier injury from C. neoformans infection. PMID: 26285576
40. Hematopoietic alpha7nAChR is important for attenuation of inflammatory responses and maintaining normal platelet reactivity, but loss of hematopoietic alpha7nAChR does not aggravate development of atherosclerosis. PMID: 25345495
41. neuroinflammation is sufficient to provoke the decrease of a7 and a4b2 nAChRs, Ab42 accumulation and memory impairment in mice and a7(1-208) nAChR-specific antibodies can cause inflammation of the brain with symptoms typical for Alzheimer disease PMID: 25816313
42. Chrna7 deficient mice were resistant to pulmonary immune hyporesponsiveness and pneumonia after stroke, demonstrating that cholinergic pathways play a pivotal role in the development of pulmonary infections after acute central nervous system injury. PMID: 26451017
43. Our study shows that alpha7nAChR modulates inflammatory response affecting the fetal brain and demonstrates its effects on offspring behavior development after MIA. PMID: 25683697
44. acute and selective blockade of alpha7nAChRs in slices from WT mice with either alpha-bungarotoxin or methyllycaconitine did not alter the expression of long-term potentiation (LTP) and long-term depression PMID: 25797465
45. Results suggest the fast nAChR current in cutaneous dorsal root ganglia neurons was carried by homomeric alpha7 nAChRs while the slow current was carried by heteromeric (most likely alpha3beta4) nAChRs PMID: 25453771
46. alpha7nAchR activation increases the phosphorylation of STAT3, reduces the expression of TNF-alpha and IL-6, and, ultimately, alleviates viral myocarditis. PMID: 25396421
47. Reduction of inflammatory response and oxidative stress play roles in alpha-7 nAchR neuro-protective effect. PMID: 25157794
48. a7 nicotinic acetylcholine receptor signaling inhibits NLRP3 inflammasome activation by preventing mitochondrial DNA release PMID: 24849809
49. Stimulation of the alpha7 nicotinic acetylcholine receptor protects against neuroinflammation after tibia fracture and endotoxemia in mice PMID: 25365546
50. Results show that NS6740 was effective in mouse models of tonic and chronic pain via alpha7 nAChR mechanisms. In addition, in vitro data suggest that the analgesic-like activity of NS6740 was not mediated by alpha7 nAChR ion channel activation. PMID: 25497451
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亞細胞定位：

Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
蛋白家族：

Ligand-gated ion channel (TC 1.A.9) family, Acetylcholine receptor (TC 1.A.9.1) subfamily, Alpha-7/CHRNA7 sub-subfamily
數據庫鏈接：

KEGG: mmu:11441

STRING: 10090.ENSMUSP00000032738

UniGene: Mm.113464