Recombinant Mouse Leucine-rich repeat-containing G-protein coupled receptor 4 (Lgr4), partial
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中文名稱：小鼠Lgr4重組蛋白
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貨號：CSB-EP012905MO-B
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說明書：
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規格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產品詳情
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純度：>85% (SDS-PAGE)
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基因名：
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Uniprot No.：
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別名：Lgr4; Gpr48Leucine-rich repeat-containing G-protein coupled receptor 4; G-protein coupled receptor 48
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種屬：Mus musculus (Mouse)
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蛋白長度：Partial
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蛋白標簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式：Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產品評價
靶點詳情
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功能：Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye. May also act as a receptor for norrin (NDP), such results however require additional confirmation in vivo. Required during spermatogenesis to activate the Wnt signaling pathway in peritubular myoid cells. Required for the maintenance of intestinal stem cells and Paneth cell differentiation in postnatal intestinal crypts. Acts as a regulator of bone formation and remodeling. Involved in kidney development; required for maintaining the ureteric bud in an undifferentiated state. Involved in the development of the anterior segment of the eye. Required during erythropoiesis. Also acts as a negative regulator of innate immunity by inhibiting TLR2/TLR4 associated pattern-recognition and proinflammatory cytokine production. Plays an important role in regulating the circadian rhythms of plasma lipids, partially through regulating the rhythmic expression of MTTP.
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基因功能參考文獻：
- he HA tag-specific antibody can be employed to characterize the biochemical features of Lgr4 and to identify possible biding partners of the protein in cells derived from various mouse tissues. PMID: 28634819
- LGR4 might play a vital role in stem cells from apical papillae proliferation and odontoblastic differentiation. PMID: 28986711
- compressive force induced the differentiation of RAW264.7 from increase in RANK and decrease in LGR4 expression. PMID: 29572179
- the host protein leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4) is essential for VSV and VSV-G pseudotyped lentivirus (VSVG-LV) to infect susceptible cells. PMID: 28842478
- Data (including data from studies using transgenic/knockout mice) suggest that LGR4 is key protein necessary for prostate cancer epithelial-mesenchymal transition and metastasis; LGR4 expression is elevated in human prostate cancer cell lines with metastatic potential; LGR4 silencing in prostate cancer cell line impairs cell migration and invasion without affecting cell proliferation. PMID: 28768769
- LGR4 is another receptor for RANKL. LGR4 competes with RANK to bind RANKL and suppresses canonical RANK signaling during osteoclast differentiation. Both whole-body (Lgr4(-/-)) and monocyte conditional knockout mice of Lgr4 (Lgr4 CKO) exhibit osteoclast hyperactivation (including elevation of osteoclast number, surface area, and size) and increased bone erosion. PMID: 27064449
- Lgr4 is a critical positive factor for skin tumorigenesis by mediating the activation of MEK1/ERK1/2 and Wnt/beta-catenin pathways. PMID: 27693558
- RSPO-LGR4/5-ZNRF3/RNF43 module controls metabolic liver zonation and is a hepatic growth/size rheostat during development, homeostasis and regeneration. PMID: 27088858
- These date indicate that hydrogen peroxide suppresses Lgr4 expression in osteoblastic cells. PMID: 27861872
- Lgr4 gene is regulated by BMP and is required for BMP effects on osteoblastic differentiation. PMID: 26332449
- Rspo1 and its receptor of leucine-rich repeat containing G-protein-coupled receptor 4 (Lgr4) should be a novel molecular signal in the transmission of mechanical stimuli to biological signal in the bone, and this signal should be in the upstream of Wnt/beta-catenin signaling for bone formation. PMID: 28272338
- Study suggests that miR-34c contributes to osteoclast differentiation by targeting LGR4, providing novel insights into understanding the molecular mechanism underlying osteoclast differentiation. PMID: 28130056
- A mechanistic understanding of RANKL-LGR4 interaction has provided new insight into LGR4 mediated RANKL signaling in osteoporosis and other diseases PMID: 27178473
- LGR4 acted as a key receptor for Rspo2 to promote osteogenesis. PMID: 27140682
- Lgr4 is critical for ovarian somatic cell specialization via the cooperative signaling of Rspo1 and Wnt/beta-catenin PMID: 26333992
- results suggest that the deletion of Lgr4 can lead to premature cataract formation, as well as progressive deterioration with aging. PMID: 25811370
- The endogenously expressed Lgr4 may act as an antagonist molecule that helps to fine-tune the R-spondin/norrin-mediated Lgr4-Wnt signaling during gonadal development. PMID: 25188337
- suggests that Lgr4 might serve as an adaptive regulator between glucose and lipid metabolism in skeletal muscle and reveals a potentially new regulator for a well-established adaptive network PMID: 26102032
- An important role for Lgr4 in motor coordination and cerebellar synaptic plasticity. PMID: 25063812
- formation of polycystic lesions and renal fibrosis induced by Gpr48 deficiency involves the activation of Wnt signaling pathway but not the TGF-beta/Smad pathway PMID: 24595031
- The members of the R-spondin family are known as activators of Wnt signaling, and Lgr4, Lgr5, and Lgr6 have been identified as receptors for R-spondins. PMID: 24616052
- Lgr4 regulates corpus luteum maturation through modulation of the WNT-mediated EGFR-ERK signaling pathway. PMID: 24877628
- LGR4 plays an important role in the regulation of plasma lipid rhythms, partially through regulating the expression of microsomal triglyceride transfer protein; these data provide a possible link between the peripheral circadian clock and lipid metabolism PMID: 24353284
- data revealed the roles of Lgr4 in early prostate development and in stem cell differentiation through regulation of the Wnt, Notch, and Sonic Hedgehog signaling pathways. PMID: 23897697
- Lgr4KO and Lgr4/5KO results in complete loss of intestinal stem cells in embryos, impairs proliferation of developing kidney and skin epithelium, and depletes Lgr5+follicular epithelium and impairs hair follicle development. PMID: 24680895
- Lgr4, which regulates eye, kidney, testis, ovary, and uterine organ development as well as mental development through genetic and epigenetic surveillance, is a novel candidate gene for the pathogenesis of AGR syndrome PMID: 24519938
- Lgr4 activates Sox2 to regulate mammary development and stem cell functions via Wnt/beta-catenin/Lef1. PMID: 23712846
- identify the transforming growth factor beta antagonist follistatin as a key mediator of RSPO-LGR4 signaling in myogenic differentiation PMID: 24344199
- LGR4 has a critical role in uterine gland morphogenesis through amplifying Wnt-beta-catenin signaling PMID: 23975934
- Multi-functional norrin is a ligand for the LGR4 receptor. PMID: 23444378
- Lgr4/Gpr48 plays a critical role in modulating the TLR2/4 signaling pathway and represents a useful therapeutic approach of targeting Lgr4/Gpr48 in TLR2/4-associated septic shock and autoimmune diseases. PMID: 23589304
- Lgr4 signaling through Wnt/beta-catenin regulates peritubular myoid cells and is essential for spermatogenesis. PMID: 23533175
- Lgr4 is critically involved in the maintenance of intestinal homeostasis and protection against inflammatory bowel disease through modulation of the Wnt/beta-catenin signaling pathway PMID: 23393138
- LGR4 is required for cell survival of the peripheral mesenchyme at the embryonic stages of nephrogenesis. PMID: 22738954
- GPR48 enhances aldosterone responsiveness by activating mineralocorticoid receptor expression. PMID: 22135314
- Lgr4 has a novel function for maintaining the ureteric bud in an undifferentiated state. PMID: 21523854
- Our results identify LGR4 as a permissive factor in the Wnt pathway in the intestine PMID: 21508962
- Relationship between the functions of Lgr4 and female reproductive systems. PMID: 20638054
- GPR48 is one of the constituents responsible for the EGFR signaling pathway with its effects directly linked to its ligand, HB-EGF. PMID: 20732323
- Gpr48 participates in the development of the male epididymis and efferent ducts through regulation of ERalpha expression via the cAMP/PKA signaling pathway. PMID: 20023170
- LGR4 is an important signaling molecule implicated in the tube morphogenesis of the male reproductive tract PMID: 16406039
- Lgr4 has pivotal roles to play in the regulation of ESR1 expression, the control of duct elongation through basement membrane remodeling, and the regional differentiation of the caput epididymidis. PMID: 17079737
- Lgr4 might be a novel gene class regulating cell motility PMID: 17850793
- Disruption of Gpr48 causes anterior segment dysgenesis through down-regulation of the Pitx2 gene. PMID: 18424556
- Inactivation of GPR48 induces the EOB phenotype by reducing epithelial cell proliferation and migration, indicating that GPR48 plays an essential role in eyelid development. PMID: 18487371
- Lgr4 might be a novel gene class regulating the development of hair follicles PMID: 18651655
- Gpr48/Lgr4 inactivation impairs definitive erythropoiesis at midgestation through down-regulation of the ATF4 signaling pathway PMID: 18955481
- Although Lgr4 hypomorphic mutant (Lgr4(Gt/Gt)) embryos developed a normal gall bladder bud at embryonic day (E) 10.25, no further elongation was observed at later stages PMID: 19301403
- Data demonstrate that Gpr48 regulates bone formation and remodeling through the cAMP-PKA-Atf4 signaling pathway. PMID: 19605502
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亞細胞定位：Cell membrane; Multi-pass membrane protein.
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蛋白家族：G-protein coupled receptor 1 family
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