Recombinant Mouse E3 ubiquitin-protein ligase MYLIP (Mylip)

中文名稱：

小鼠Mylip重組蛋白
貨號：

CSB-YP807377MO
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Mylip重組蛋白
貨號：

CSB-EP807377MO
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Mylip重組蛋白
貨號：

CSB-EP807377MO-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Mylip重組蛋白
貨號：

CSB-BP807377MO
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Mylip重組蛋白
貨號：

CSB-MP807377MO
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Mylip
Uniprot No.：

Q8BM54
別名：

Mylip; E3 ubiquitin-protein ligase MYLIP; EC 2.3.2.27; Inducible degrader of the LDL-receptor; Idol; Myosin regulatory light chain-interacting protein; MIR; RING-type E3 ubiquitin transferase MYLIP
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-445
氨基酸序列

MLCYVTRPDA VLMEVEVEAK ANGEDCLNQV CRRLGIIEVD YFGLQFTGSK GESLWLNLRN RISQQMDGLA PYRLKLRVKF FVEPHLILQE QTRHIFFLHI KESLLAGHLQ CSPEQAVELS ALLAQTKFGD YNQNTAQYSY EDLCEKELSS STLNSIVAKH KELEGISQAS AEYQVLQIVS AMENYGIEWH AVRDSEGQKL LIGVGPEGIS ICKEDFSPIN RIAYPVVQMA TQSGKNVYLT VTKESGNSIV LLFKMISTRA ASGLYRAITE THAFYRCDTV TSAVMMQYSR DLKGHLASLF LNENINLGKK YVFDIKRTSK EVYDHARRAL YNAGVVDLVS RSDQSPPSSP LKSSDSSMSC SSCEGLSCQQ TRVLQEKLRK LKEAMLCMAC CEEEINSTFC PCGHTVCCES CAAQLQSCPV CRSRVEHVQH VYLPTHTSLL NLTVI
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
基因功能參考文獻：
1. describe the recently generated mouse model, L-sIDOL Tg mice, which express a dominant active form of Inducible Degrader Of the Low-density lipoprotein receptor (IDOL) in a liver-specific manner. This murine model offers significant advantages over previously established models for the study of hypercholesterolemia and atherosclerosis. PMID: 28205167
2. the effects caused by human inducible degrader of the low-density lipoprotein expression are LDLR- dependent given the unchanged plasma lipids in LAhB mice lacking low-density lipoprotein receptor PMID: 26786161
3. Suggest Idol as a gatekeeper of LDLR-dependent ApoE and Abeta clearance in the brain and a potential enzyme target for therapeutic intervention in Alzheimer disease. PMID: 26582899
4. Estradiol-treatment led to impaired contractile function in male cardiomyocytes only, which was characterized by increased Mylip mRNA and protein levels, and decreased myosin regulatory light chain protein. PMID: 22261164
5. These data identify the IDOL-LDLR interaction as an evolutionarily conserved mechanism for the regulation of lipid uptake and suggest that this interaction could potentially be exploited for the pharmacologic modulation of lipid metabolism. PMID: 22109552
6. Data show that the identification of VLDLR and ApoER2 as Idol(Mylip) targets suggests potential roles for this LXR-inducible E3 ligase in the central nervous system in addition to lipid metabolism. PMID: 20427281
7. study shows the LXR-Idol(Mylip)-LDLR axis defines a complementary pathway to sterol response element-binding proteins for sterol regulation of cholesterol uptake PMID: 19520913
顯示更多

收起更多
亞細胞定位：

Cytoplasm. Cell membrane; Peripheral membrane protein.
組織特異性：

Expressed in liver, spleen, intestine and adrenals.
數據庫鏈接：

KEGG: mmu:218203

STRING: 10090.ENSMUSP00000047403

UniGene: Mm.212855