Recombinant Mouse Dihydroorotate dehydrogenase (quinone),mitochondrial (Dhodh), partial

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中文名稱：

小鼠Dhodh重組蛋白
貨號：

CSB-EP006852MO1
說明書：

MSDS Datasheet
規(guī)格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

Dhodh
Uniprot No.：

O35435
別名：

Dhodh; Dihydroorotate dehydrogenase; quinone, mitochondrial; DHOdehase; Dihydroorotate oxidase
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
來源：

E.coli
分子量：

45.0 kDa
表達(dá)區(qū)域：

31-395aa
氨基酸序列

TGDDHFYAEYLMPALQRLLDPESAHRLAVRVISLGLLPRATFQDSNMLEVRVLGHKFRNPVGIAAGFDKHGEAVDGLYKLGFGFVEVGSVTPQPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSAVEHRLRARQQKQTQLTTDGLPLGINLGKNKTSVDAAADYVEGVRILGPLADYLVVNVSSPNTAGLRSLQGKTELRRLLSKVLQERDALKGPQKPAVLVKIAPDLTAQDKEDIASVARELGIDGLIITNTTVSRPVGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGTIPIIGVGGVSSGQDALEKIQAGASLVQLYTALTFLGPPVVARVKRELEALLKERGFNTVTDAIGVDHRR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Tris-based buffer,50% glycerol
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

The preparation of Recombinant Mouse Dhodh protein included 3 main steps: construct the expression vector, expression of protein of interest, and protein purification. Every step was performed under a strict QC system so that we got the premium protein. This Dhodh was expressed in E.coli at and fused with N-terminal 6xHis tag. According to SDS-PAGE, the purity turns out to be 85%+.
Dhodh is a key enzyme in de novo pyrimidine biosynthesis. the expression level of dihydroorotate dehydrogenase has been reported to be elevated in various types of malignant tumors and its tumor-promoting effect was considered to relate to its pyrimidine synthesis function. Dhodh is the rate-limiting enzyme in the uridine monophosphate (UMP) biosynthetic pathway and mostly located in the inner membrane of mitochondria, catalyzing the transformation of dihydroorotate to orotate dependent of Mg2+ and ATP. Mutations of Dhodh have been associated with various genetic diseases. For example, Dhodh G202A, R346W causes deficient protein stability and R135C impairs the enzymatic activity, which are linked to Miller syndrome. Dhodh polymorphism was reported to be linked with rheumatoid arthritis and lung cancer as well. Additionally, enhanced activity of DHODH has been implicated as a biomarker of malignant tumor including gastric cancer and skin cancer.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
基因功能參考文獻(xiàn)：
1. DHODH inhibition led to a marked decrease in melanoma growth both in vitro and in mouse xenograft studies PMID: 21430780
2. immunosuppressive agent HR325 (an inhibitor of dihydroorotate dehydrogenase, DHODH) inhibits immunoglobulin (Ig) secretion both in vitro and in vivo and that this effect can be reverse with exogenous uridine PMID: 12100469
3. Dihydroorotate dehydrogenase mRNA and protein expression analysis in normal and drug-resistant cells of different species PMID: 15571246
亞細(xì)胞定位：

Mitochondrion inner membrane; Single-pass membrane protein.
蛋白家族：

Dihydroorotate dehydrogenase family, Type 2 subfamily
數(shù)據(jù)庫鏈接：

KEGG: mmu:56749

STRING: 10090.ENSMUSP00000070303

UniGene: Mm.23894