Recombinant Mouse DNA topoisomerase 2-beta (Top2b), partial

中文名稱：

小鼠Top2b重組蛋白
貨號：

CSB-YP717547MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Top2b重組蛋白
貨號：

CSB-EP717547MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Top2b重組蛋白
貨號：

CSB-EP717547MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Top2b重組蛋白
貨號：

CSB-BP717547MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Top2b重組蛋白
貨號：

CSB-MP717547MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Top2b
Uniprot No.：

Q64511
別名：

Top2b; DNA topoisomerase 2-beta; EC 5.6.2.2; DNA topoisomerase II; beta isozyme
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand.
基因功能參考文獻：
1. Top2beta controls spinal motor neurons (MN) migration and connectivity; is not required for MN generation or survival; has a selective role in columnar specification. In absence of Top2beta, phrenic MN identity is eroded, while other motor columns are partially preserved but fail to cluster to their proper position. In Top2beta-/- mice, peripheral connectivity is impaired as MNs exhibit a profound deficit in terminal b... PMID: 29379870
2. our data establish a combinatorial cell-autonomous and non-cell-autonomous role for Top2b in the late stage of photoreceptor differentiation and maturation PMID: 28370415
3. We demonstrated that Nup98-TopIIbeta and Nup98-SETBP1 negatively regulate the XPO1-mediated protein export. Our results will contribute to the understanding of the molecular mechanism by which the Nup98-fusion proteins induce tumorigenesis. PMID: 28392395
4. TOP2beta as a factor involved in regulating granulosa cell genomic integrity and follicle atresia. PMID: 24002654
5. Results identified topoisomerase IIbeta (Top2beta) as a novel determinant for camptothecin sensitivity. PMID: 23344961
6. Top IIbeta might be a down-stream target of Nurr1, which might influence the processes of axon genesis in dopaminergic neurons via the regulation of TOP IIbeta expression. PMID: 22296971
7. Findings suggest a chromatin-based targeting of Top2beta to regulatory regions in the genome to govern the transcriptional program associated with neuronal differentiation and longevity. PMID: 22474351
8. Data suggest that activity of TOP2B in elongating spermatids is strongly inhibited by PARP1 and PARP2, and this is in turn counteracted by the PAR-degrading activity of PAR glycohydrolase. PMID: 21228215
9. proteasomal degradation of TOP2beta induced by the TOP2-DNA covalent complex or the TOP2 circular clamp is due to transcriptional arrest but not DNA damage PMID: 12629207
10. DNA topoisomerase II beta deficiency severely affects cerebral cortex stratification: no subplate is discernible, and neurons born at later stages of corticogenesis fail to migrate to the superficial layers. PMID: 12773624
11. A nuclear matrix-associated topoisomerase IIB (TOP2B) interacting with an extracellular Mn2+/Ca2+-dependent nuclease cleaves sperm DNA into loop-sized fragments. PMID: 16914690
12. Results support a role of topoisomerase IIbeta in activation/repression of developmentally regulated genes at late stages of neuronal differentiation. PMID: 16923961
13. These results suggest that the defect in establishing neuromuscular junctions in top2beta knockout mice could be due to the lack of TopIIbeta-mediated neurite outgrowth. PMID: 17493591
14. VP-16-induced carcinogenesis involves mainly the beta rather than the alpha isozyme of Top2; VP-16-induced DNA sequence rearrangements and double-strand breaks are found to be Top2beta-dependent PMID: 17578914
15. NRF-1 can also directly interact with poly(ADP-ribose) polymerase 1 (PARP-1) and co-purify the PARP-1.DNA-PK.Ku80.Ku70.topoisomerase IIbeta-containing protein complex. PMID: 19181665
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亞細胞定位：

Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus.
蛋白家族：

Type II topoisomerase family
數據庫鏈接：

KEGG: mmu:21974

STRING: 10090.ENSMUSP00000017629

UniGene: Mm.130362